Detailed information of TA system TA112017

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): cptAB/Cpta(toxin)
• Location: 3320919..3321579
• Replicon: chromosome
• Accession: NZ_CP032619
• Organism: Pectobacterium carotovorum strain HG-49

Toxin (Protein)

• Gene name: cptA
• Uniprot ID: -
• Locus tag: D7Z95_RS14995
• Protein ID: WP_043881709.1
• Coordinates: 3321205..3321579 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: cptB
• Uniprot ID: C6D8Y5
• Locus tag: D7Z95_RS14990
• Protein ID: WP_012773340.1
• Coordinates: 3320919..3321185 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D7Z95_RS14970	3316911..3317915	-	1005	WP_205947074.1	LacI family DNA-binding transcriptional regulator	-
D7Z95_RS14975	3317959..3318567	-	609	WP_181829659.1	HD domain-containing protein	-
D7Z95_RS14980	3318980..3319627	+	648	WP_012773338.1	hemolysin III family protein	-
D7Z95_RS14985	3319682..3320683	-	1002	WP_205947073.1	tRNA-modifying protein YgfZ	-
D7Z95_RS14990	3320919..3321185	+	267	WP_012773340.1	FAD assembly factor SdhE	Antitoxin
D7Z95_RS14995	3321205..3321579	+	375	WP_043881709.1	protein YgfX	Toxin
D7Z95_RS15000	3321649..3322584	+	936	WP_205947072.1	23S rRNA (adenine(1618)-N(6))-methyltransferase RlmF	-
D7Z95_RS15005	3322630..3322953	-	324	WP_180777498.1	hypothetical protein	-
D7Z95_RS15010	3322982..3323500	-	519	WP_012773344.1	flavodoxin FldB	-
D7Z95_RS15015	3323663..3324991	-	1329	WP_012773346.1	MFS transporter	-
D7Z95_RS15020	3325303..3326202	+	900	WP_012773347.1	site-specific tyrosine recombinase XerD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Inner membrane protein YgfX (2-119)

Antitoxin

• Flavinator of succinate dehydrogenase (8-79)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 14673.34 Da
• Isoelectric Point: 10.6585

>T112017 WP_043881709.1 NZ_CP032619:3321205-3321579 [Pectobacterium carotovorum]
MQLLSLIVHGLLVLLILLAPWPDGYAWLWLCLVTMVMFGFIRSQRNIKSRQGEIVLLSETTLNWRQQEWQIVKRPWLLKN
GVLLSLQAVNGKDRQQLWLASDSMGDDEWRHLRQLLLQQKNWAR

Nucleotide

• Length: 375 bp

>T112017 NZ_CP032619:3321205-3321579 [Pectobacterium carotovorum]
ATGCAACTGCTTTCATTGATTGTGCATGGCCTATTAGTATTGCTCATTCTTCTGGCGCCGTGGCCGGACGGCTATGCATG
GCTGTGGCTATGCCTGGTCACGATGGTGATGTTCGGTTTTATCCGTAGCCAAAGGAATATCAAATCCCGGCAGGGGGAAA
TCGTTTTGCTCAGCGAAACGACCCTGAACTGGCGGCAGCAGGAATGGCAGATCGTTAAACGACCGTGGTTGCTGAAAAAT
GGCGTATTGCTGTCATTGCAGGCAGTGAACGGTAAAGACAGACAGCAGCTGTGGCTGGCATCGGACAGCATGGGCGATGA
CGAATGGCGTCATCTGCGTCAGCTTCTTTTGCAGCAGAAAAACTGGGCGAGATAG

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10465.94 Da
• Isoelectric Point: 6.2223

>AT112017 WP_012773340.1 NZ_CP032619:3320919-3321185 [Pectobacterium carotovorum]
MEIDNKSRIHWACRRGMRELDIAIMPFFEHDYDTLSDSDKRTFVRLLQSDDPDLFNWLMNHGEPKDGELKRMISLIQTRN
KDRGPVAM

Nucleotide

• Length: 267 bp

>AT112017 NZ_CP032619:3320919-3321185 [Pectobacterium carotovorum]
ATGGAAATCGATAATAAATCACGCATTCATTGGGCATGCCGCCGGGGTATGCGTGAGTTGGATATCGCGATCATGCCGTT
TTTTGAGCATGACTACGACACACTCAGTGACAGCGATAAGCGCACCTTTGTGCGCCTGTTACAAAGCGATGATCCCGATT
TATTCAACTGGCTGATGAACCACGGCGAGCCGAAAGACGGAGAGCTGAAACGCATGATTTCTCTTATTCAAACGCGAAAT
AAAGATCGTGGCCCAGTGGCAATGTGA

Structures

Antitoxin

• AlphaFold DB: A0A7W2E501