Detailed information of TA system TA112016

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 3150467..3150993
• Replicon: chromosome
• Accession: NZ_CP032619
• Organism: Pectobacterium carotovorum strain HG-49

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: -
• Locus tag: D7Z95_RS14195
• Protein ID: WP_205947126.1
• Coordinates: 3150467..3150772 (-)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: C6C380
• Locus tag: D7Z95_RS14200
• Protein ID: WP_015855076.1
• Coordinates: 3150775..3150993 (-)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D7Z95_RS14175	3145558..3147681	-	2124	WP_205947129.1	ATP-binding cassette domain-containing protein	-
D7Z95_RS14180	3147686..3148768	-	1083	WP_205947128.1	ThiF family adenylyltransferase	-
D7Z95_RS14185	3148752..3149888	-	1137	WP_240346548.1	ThiF family adenylyltransferase	-
D7Z95_RS14190	3149944..3150153	-	210	WP_205947127.1	hypothetical protein	-
D7Z95_RS14195	3150467..3150772	-	306	WP_205947126.1	type II toxin-antitoxin system toxin CcdB	Toxin
D7Z95_RS14200	3150775..3150993	-	219	WP_015855076.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
D7Z95_RS14205	3151052..3151795	-	744	WP_205947125.1	MobC family replication-relaxation protein	-
D7Z95_RS14210	3151893..3152243	+	351	WP_205947124.1	hypothetical protein	-
D7Z95_RS14215	3152699..3153040	-	342	WP_205947123.1	hypothetical protein	-
D7Z95_RS14220	3153181..3153351	+	171	WP_164669135.1	hypothetical protein	-
D7Z95_RS14225	3153497..3154756	-	1260	WP_205947122.1	tyrosine-type recombinase/integrase	-
D7Z95_RS14230	3154865..3155047	-	183	WP_264040170.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	3143285..3164516	21231

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-100)

Antitoxin

• Post-segregation antitoxin CcdA (2-72)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11764.67 Da
• Isoelectric Point: 4.6761

>T112016 WP_205947126.1 NZ_CP032619:c3150772-3150467 [Pectobacterium carotovorum]
MQFIVYEYKRASHYKMFVDVQSDIVDTPKRRMVIPLIEAHHLSEKVNKTLFPLIRIDGEDYRLMTTELSSVPVEVIGEAI
MDLGDYADEIKDAINLMFWGI

Nucleotide

• Length: 306 bp

>T112016 NZ_CP032619:c3150772-3150467 [Pectobacterium carotovorum]
ATGCAATTCATTGTTTATGAATATAAACGCGCCAGCCACTACAAAATGTTTGTCGATGTGCAAAGCGATATTGTCGACAC
ACCAAAGCGGCGCATGGTCATCCCGCTTATCGAAGCACATCATCTGTCTGAGAAAGTGAATAAAACATTGTTCCCGCTGA
TTCGCATCGATGGGGAAGATTATCGGCTGATGACCACAGAGCTGTCGAGTGTTCCTGTTGAGGTGATTGGTGAGGCTATC
ATGGATCTTGGAGACTATGCCGATGAGATCAAGGATGCTATTAATCTTATGTTTTGGGGGATTTGA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8178.13 Da
• Isoelectric Point: 7.0135

>AT112016 WP_015855076.1 NZ_CP032619:c3150993-3150775 [Pectobacterium carotovorum]
MKHRVSVTVDKDNYQVLSAAGVNISGLVNDAIGKEARRIKAEEWKKENREGMEEVARFIAQNGSFADENRNW

Nucleotide

• Length: 219 bp

>AT112016 NZ_CP032619:c3150993-3150775 [Pectobacterium carotovorum]
ATGAAACACCGCGTCAGCGTTACCGTGGACAAAGACAATTATCAGGTTCTGAGTGCTGCCGGAGTCAATATTTCCGGGCT
GGTGAATGACGCCATTGGCAAAGAAGCCCGCCGCATTAAGGCTGAGGAGTGGAAAAAGGAAAACCGCGAAGGGATGGAAG
AAGTCGCCCGATTTATCGCGCAGAATGGCTCCTTTGCGGATGAAAACAGGAACTGGTGA

Structures

Antitoxin

• AlphaFold DB: A0A250BG46