Detailed information of TA system TA112013

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): stbDE/ParE-DnaT
• Location: 2911009..2911540
• Replicon: chromosome
• Accession: NZ_CP032619
• Organism: Pectobacterium carotovorum strain HG-49

Toxin (Protein)

• Gene name: stbE
• Uniprot ID: A0A1V2R9S3
• Locus tag: D7Z95_RS13130
• Protein ID: WP_012822123.1
• Coordinates: 2911253..2911540 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: stbD
• Uniprot ID: -
• Locus tag: D7Z95_RS13125
• Protein ID: WP_180778477.1
• Coordinates: 2911009..2911263 (+)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D7Z95_RS13100	2906144..2906734	-	591	WP_012773024.1	DnaA initiator-associating protein DiaA	-
D7Z95_RS13105	2906798..2907193	-	396	WP_181830009.1	YraN family protein	-
D7Z95_RS13110	2907151..2909169	-	2019	WP_181829974.1	penicillin-binding protein activator	-
D7Z95_RS13115	2909231..2910118	+	888	WP_205947215.1	16S rRNA (cytidine(1402)-2'-O)-methyltransferase	-
D7Z95_RS13125	2911009..2911263	+	255	WP_180778477.1	plasmid stabilization protein	Antitoxin
D7Z95_RS13130	2911253..2911540	+	288	WP_012822123.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
D7Z95_RS13135	2911719..2912504	+	786	WP_012773028.1	4,5-DOPA dioxygenase extradiol	-
D7Z95_RS13140	2912574..2913734	-	1161	WP_180778474.1	glutathionylspermidine synthase family protein	-
D7Z95_RS13145	2913746..2914420	-	675	WP_012773030.1	DUF1190 family protein	-
D7Z95_RS13150	2914687..2916078	-	1392	WP_205947214.1	outer membrane channel protein TolC	-
D7Z95_RS13155	2916213..2916368	-	156	WP_012773032.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-82)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 10997.94 Da
• Isoelectric Point: 10.7694

>T112013 WP_012822123.1 NZ_CP032619:2911253-2911540 [Pectobacterium carotovorum]
MTYKLKFVPSAMKEWKKLGHPVREQFKKKLVERLENPRVPSAQLHGRKDQYKIKLKSAGYRLVYLVQDETITVMVMGVGK
REGSQVYSDTKGRSA

Nucleotide

• Length: 288 bp

>T112013 NZ_CP032619:2911253-2911540 [Pectobacterium carotovorum]
ATGACCTATAAGTTAAAATTTGTGCCTTCTGCAATGAAGGAATGGAAAAAATTAGGGCATCCTGTCAGAGAACAATTCAA
AAAAAAGCTAGTTGAGCGTTTAGAAAATCCTCGTGTTCCTTCAGCCCAGCTCCATGGTCGTAAGGACCAGTATAAAATTA
AACTGAAGTCTGCTGGTTACAGGCTGGTGTATTTGGTTCAGGATGAGACCATTACGGTGATGGTCATGGGGGTTGGAAAG
CGCGAAGGTAGTCAGGTTTATTCTGATACTAAAGGTCGCTCTGCTTAA

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9240.44 Da
• Isoelectric Point: 3.7947

>AT112013 WP_180778477.1 NZ_CP032619:2911009-2911263 [Pectobacterium carotovorum]
MAFNILTNTAASITELKRDPMGTVRAAEGETIAILNRNEPAFYCVPPAMFAYLMELAEDAELGRTVDDRMSELADAEEIS
LDDL

Nucleotide

• Length: 255 bp

>AT112013 NZ_CP032619:2911009-2911263 [Pectobacterium carotovorum]
ATGGCTTTCAATATCCTAACTAACACAGCGGCCAGTATCACTGAACTGAAACGCGACCCAATGGGGACAGTACGGGCTGC
TGAAGGTGAGACTATCGCTATACTGAACCGGAATGAGCCTGCGTTCTATTGTGTGCCTCCCGCCATGTTCGCTTATTTAA
TGGAATTGGCTGAAGATGCAGAGCTGGGGCGTACTGTTGATGATCGTATGTCAGAGTTAGCTGACGCGGAAGAGATTTCG
CTCGATGACCTATAA

Structures

Toxin

• AlphaFold DB: A0A1V2R9S3