Detailed information of TA system TA112010

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 2291714..2292348
• Replicon: chromosome
• Accession: NZ_CP032619
• Organism: Pectobacterium carotovorum strain HG-49

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: D7Z95_RS10415
• Protein ID: WP_181845733.1
• Coordinates: 2292073..2292348 (-)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: D7Z95_RS10410
• Protein ID: WP_205947374.1
• Coordinates: 2291714..2292076 (-)
• Length: 121 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D7Z95_RS10395	2288036..2288998	-	963	WP_012772882.1	6-phosphofructokinase	-
D7Z95_RS10400	2289206..2290108	-	903	WP_012772881.1	CDF family cation-efflux transporter FieF	-
D7Z95_RS10405	2290152..2291339	-	1188	WP_205947375.1	sugar transporter	-
D7Z95_RS10410	2291714..2292076	-	363	WP_205947374.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
D7Z95_RS10415	2292073..2292348	-	276	WP_181845733.1	type II toxin-antitoxin system HicA family toxin	Toxin
D7Z95_RS10420	2292584..2293108	-	525	WP_012772879.1	cell-envelope stress modulator CpxP	-
D7Z95_RS10425	2293262..2293960	+	699	WP_012772878.1	envelope stress response regulator transcription factor CpxR	-
D7Z95_RS10430	2293957..2295327	+	1371	WP_010281575.1	envelope stress sensor histidine kinase CpxA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	2286489..2287508	1019

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (30-80)

Antitoxin

• Uncharacterised protein family HicB (61-113)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10051.64 Da
• Isoelectric Point: 11.1875

>T112010 WP_181845733.1 NZ_CP032619:c2292348-2292073 [Pectobacterium carotovorum]
MQGKIASLRKKQRETLSQIFKTPVLSGVKWSNVESLITALGGEIKEGSGSRVRFLLNGSIARFHRPHPSPDTDKGALVNL
REWLESIGVKP

Nucleotide

• Length: 276 bp

>T112010 NZ_CP032619:c2292348-2292073 [Pectobacterium carotovorum]
ATGCAGGGAAAAATAGCCAGTCTACGAAAAAAGCAGCGAGAAACGCTGAGCCAAATTTTCAAAACACCCGTGCTTTCAGG
TGTTAAATGGTCAAATGTTGAGTCACTCATCACTGCGTTAGGTGGTGAAATTAAGGAAGGTAGCGGGTCACGGGTGCGAT
TTTTATTGAATGGGAGTATCGCCCGGTTCCATCGTCCTCACCCGTCACCGGATACTGATAAAGGCGCATTGGTTAACCTG
CGTGAATGGTTGGAAAGTATAGGAGTAAAACCATGA

Antitoxin

Protein

• Length: 121 a.a.
• Molecular weight: 13567.37 Da
• Isoelectric Point: 4.4981

>AT112010 WP_205947374.1 NZ_CP032619:c2292076-2291714 [Pectobacterium carotovorum]
MTKALNTPNTMVVSGQSAIISYVPEIGMFRGKFIGLSGYCDFVADSINGLRNEGEISLREYLEDCQENGIEPYEREEKVK
TFTLRYPESFGERLTIAAAERQISVNTFIVETLNERMKQA

Nucleotide

• Length: 363 bp

>AT112010 NZ_CP032619:c2292076-2291714 [Pectobacterium carotovorum]
ATGACTAAAGCACTTAACACACCAAATACGATGGTTGTTTCCGGCCAATCGGCCATTATCAGCTATGTACCGGAAATCGG
TATGTTTCGCGGTAAGTTTATTGGCTTATCTGGCTACTGTGATTTTGTCGCTGACAGTATTAATGGGCTGAGAAACGAGG
GCGAAATTTCGCTACGTGAATATCTGGAAGACTGTCAGGAAAATGGCATTGAACCTTACGAACGTGAAGAGAAGGTGAAA
ACGTTCACTCTCCGTTATCCTGAATCATTCGGAGAACGACTGACTATTGCGGCGGCTGAACGTCAAATTTCAGTGAATAC
ATTTATTGTCGAAACGCTTAATGAACGTATGAAACAGGCTTAA