Detailed information of TA system TA112009

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 2253405..2254022
• Replicon: chromosome
• Accession: NZ_CP032619
• Organism: Pectobacterium carotovorum strain HG-49

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: C6DHN3
• Locus tag: D7Z95_RS10240
• Protein ID: WP_010281507.1
• Coordinates: 2253405..2253584 (+)
• Length: 60 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: D7Z95_RS10245
• Protein ID: WP_180779155.1
• Coordinates: 2253612..2254022 (+)
• Length: 137 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D7Z95_RS10215	2248650..2249810	-	1161	WP_012772912.1	cystathionine gamma-synthase	-
D7Z95_RS10220	2249998..2250315	+	318	WP_005973355.1	met regulon transcriptional regulator MetJ	-
D7Z95_RS10225	2250531..2251328	+	798	WP_205947384.1	ABC transporter ATP-binding protein	-
D7Z95_RS10230	2251338..2252300	+	963	WP_205947383.1	ABC transporter substrate-binding protein	-
D7Z95_RS10235	2252297..2253307	+	1011	WP_205947382.1	iron ABC transporter permease	-
D7Z95_RS10240	2253405..2253584	+	180	WP_010281507.1	type II toxin-antitoxin system HicA family toxin	Toxin
D7Z95_RS10245	2253612..2254022	+	411	WP_180779155.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
D7Z95_RS10250	2254149..2254364	-	216	WP_012772907.1	50S ribosomal protein L31	-
D7Z95_RS10255	2254679..2256877	+	2199	WP_181847420.1	primosomal protein N'	-
D7Z95_RS10260	2257154..2258197	+	1044	WP_012772905.1	DNA-binding transcriptional regulator CytR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (4-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-128)

Sequences

Toxin

Protein

• Length: 60 a.a.
• Molecular weight: 6560.69 Da
• Isoelectric Point: 10.7838

>T112009 WP_010281507.1 NZ_CP032619:2253405-2253584 [Pectobacterium carotovorum]
MDSRTLIAEIKADGWELIRVNGSHHHFMHPSKPGLVTIPHPKKDLPIGTVKSIRKQAGI

Nucleotide

• Length: 180 bp

>T112009 NZ_CP032619:2253405-2253584 [Pectobacterium carotovorum]
ATGGATAGCAGGACGTTAATCGCAGAAATCAAGGCCGATGGGTGGGAGCTGATACGAGTGAACGGAAGCCACCACCATTT
TATGCACCCCAGCAAACCGGGATTAGTCACAATCCCCCACCCCAAGAAGGATTTACCAATAGGTACGGTAAAAAGCATCA
GGAAACAGGCAGGAATATGA

Antitoxin

Protein

• Length: 137 a.a.
• Molecular weight: 14492.36 Da
• Isoelectric Point: 4.3391

>AT112009 WP_180779155.1 NZ_CP032619:2253612-2254022 [Pectobacterium carotovorum]
MFYPIAIEAGDDTHAYGVTVPDLPGCFSAGDTLDDAIANAKEAITGHIELLVEMGQDIPTVSTVGQLAKGSEYAGYTWAV
VDIDVTRLMGGSEKINVTLPKSLIDRIDRCVASNPEFKSRSGFLAQAALERISSSR

Nucleotide

• Length: 411 bp

>AT112009 NZ_CP032619:2253612-2254022 [Pectobacterium carotovorum]
ATGTTTTATCCCATTGCAATTGAAGCAGGCGACGATACTCACGCATACGGCGTAACAGTACCAGATTTACCGGGCTGTTT
TTCTGCAGGCGATACGCTAGACGATGCTATCGCAAACGCTAAAGAAGCGATTACCGGACATATCGAGCTACTGGTTGAGA
TGGGTCAGGATATTCCCACCGTATCAACAGTAGGTCAGTTGGCAAAAGGGTCTGAGTATGCAGGGTACACGTGGGCGGTT
GTTGATATTGATGTAACTCGTTTAATGGGCGGCTCAGAAAAAATTAACGTTACATTGCCCAAATCGCTGATTGACCGCAT
TGATCGTTGCGTGGCGAGTAATCCAGAATTTAAAAGTCGTTCGGGTTTTCTTGCACAGGCGGCATTAGAGCGAATTTCGT
CTTCCCGATGA

Structures

Toxin

• AlphaFold DB: A0A7W2I1S0