Detailed information of TA system TA112005

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA(toxin)
• Location: 2237524..2238137
• Replicon: chromosome
• Accession: NZ_CP032618
• Organism: Pseudomonas fluorescens strain PF08

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: D7M10_RS10415
• Protein ID: WP_120732485.1
• Coordinates: 2237886..2238137 (-)
• Length: 84 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: D7M10_RS10410
• Protein ID: WP_120732483.1
• Coordinates: 2237524..2237889 (-)
• Length: 122 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D7M10_RS10390	2232928..2234079	+	1152	WP_057439103.1	HlyD family secretion protein	-
D7M10_RS10395	2234069..2235589	+	1521	WP_057959158.1	multidrug efflux MFS transporter	-
D7M10_RS10400	2235623..2236207	-	585	WP_057959159.1	TetR/AcrR family transcriptional regulator	-
D7M10_RS10405	2236350..2237522	+	1173	WP_120732481.1	MFS transporter	-
D7M10_RS10410	2237524..2237889	-	366	WP_120732483.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
D7M10_RS10415	2237886..2238137	-	252	WP_120732485.1	type II toxin-antitoxin system HicA family toxin	Toxin

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (20-76)

Antitoxin

• Uncharacterised protein family HicB (55-105)

Sequences

Toxin

Protein

• Length: 84 a.a.
• Molecular weight: 9477.06 Da
• Isoelectric Point: 9.9512

>T112005 WP_120732485.1 NZ_CP032618:c2238137-2237886 [Pseudomonas fluorescens]
VSKNEKLLAKLLNLQMAFTWPELVTLLGWRGYRQIEGAGSRVKFDIGDPSAMITLHKPHPGNELKYYIRRQIIEQLESGG
LIQ

Nucleotide

• Length: 252 bp

>T112005 NZ_CP032618:c2238137-2237886 [Pseudomonas fluorescens]
GTGTCCAAAAATGAAAAGCTGCTCGCCAAACTGCTTAACCTGCAGATGGCATTTACCTGGCCTGAGCTCGTGACATTGTT
AGGCTGGCGGGGTTACCGACAAATCGAAGGGGCTGGGAGCCGCGTCAAGTTCGACATAGGTGACCCGAGTGCAATGATCA
CCTTGCACAAGCCCCACCCTGGCAATGAACTGAAGTACTACATTCGGCGCCAGATCATTGAACAGTTGGAATCAGGAGGC
TTGATTCAGTGA

Antitoxin

Protein

• Length: 122 a.a.
• Molecular weight: 13299.11 Da
• Isoelectric Point: 7.4017

>AT112005 WP_120732483.1 NZ_CP032618:c2237889-2237524 [Pseudomonas fluorescens]
VSNQLKHKGYIGSIEASVEDNCLFGKLLFIRPLVSYEGQTVAQLEAAFREAVEDYLATCQTLGQAPEKPCKGSFNVRVGH
DLHLAAALAATQKKVTLNDLTRQALNEFLRHHRTDSHPALG

Nucleotide

• Length: 366 bp

>AT112005 NZ_CP032618:c2237889-2237524 [Pseudomonas fluorescens]
GTGAGCAACCAGTTGAAACACAAAGGTTACATCGGCTCCATAGAAGCCAGCGTCGAGGACAATTGCCTGTTCGGCAAGCT
GCTGTTTATCCGCCCCCTGGTCAGTTACGAAGGGCAGACCGTGGCCCAGTTGGAGGCCGCCTTTCGCGAGGCCGTGGAGG
ATTATCTGGCTACTTGCCAGACCCTGGGCCAGGCACCGGAAAAGCCCTGCAAAGGCTCCTTCAATGTACGGGTCGGCCAT
GATCTGCACCTGGCTGCGGCCCTGGCGGCGACGCAGAAAAAGGTCACGCTCAACGACCTGACACGCCAGGCGCTGAACGA
GTTCCTGCGCCATCACCGTACAGACAGCCACCCCGCCCTGGGGTGA