Detailed information of TA system TA112004

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 1814749..1815366
• Replicon: chromosome
• Accession: NZ_CP032618
• Organism: Pseudomonas fluorescens strain PF08

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: D7M10_RS08465
• Protein ID: WP_057438840.1
• Coordinates: 1814749..1814937 (+)
• Length: 63 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: D7M10_RS08470
• Protein ID: WP_057438841.1
• Coordinates: 1814962..1815366 (+)
• Length: 135 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D7M10_RS08435	1809855..1810697	+	843	WP_057438836.1	alpha-D-ribose 1-methylphosphonate 5-phosphate C-P-lyase PhnJ	-
D7M10_RS08440	1810658..1811467	+	810	WP_120734408.1	phosphonate C-P lyase system protein PhnK	-
D7M10_RS08445	1811464..1812186	+	723	WP_120732262.1	phosphonate C-P lyase system protein PhnL	-
D7M10_RS08450	1812176..1813321	+	1146	WP_057438838.1	alpha-D-ribose 1-methylphosphonate 5-triphosphate diphosphatase	-
D7M10_RS08455	1813321..1813887	+	567	WP_057444570.1	phosphonate metabolism protein/1,5-bisphosphokinase (PRPP-forming) PhnN	-
D7M10_RS08460	1813878..1814627	+	750	WP_057958969.1	phosphonate metabolism protein PhnP	-
D7M10_RS08465	1814749..1814937	+	189	WP_057438840.1	type II toxin-antitoxin system HicA family toxin	Toxin
D7M10_RS08470	1814962..1815366	+	405	WP_057438841.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
D7M10_RS08475	1815725..1817239	+	1515	WP_162947790.1	M10 family metallopeptidase C-terminal domain-containing protein	-
D7M10_RS08480	1817263..1818459	-	1197	WP_057438842.1	MFS transporter	-
D7M10_RS08485	1818690..1819694	-	1005	WP_057438843.1	sulfate ABC transporter substrate-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (6-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-127)

Sequences

Toxin

Protein

• Length: 63 a.a.
• Molecular weight: 7132.31 Da
• Isoelectric Point: 11.4962

>T112004 WP_057438840.1 NZ_CP032618:1814749-1814937 [Pseudomonas fluorescens]
VQSRLLIKELQEAGWVLDRVMGSHHIFTHRYNPSTIPVPHPKKDLPLGTVRSIRQRAGLFSF

Nucleotide

• Length: 189 bp

>T112004 NZ_CP032618:1814749-1814937 [Pseudomonas fluorescens]
GTGCAAAGCAGGCTATTGATCAAGGAACTGCAAGAGGCGGGCTGGGTGTTGGATCGGGTCATGGGCAGCCATCACATTTT
CACCCATCGCTATAACCCGTCCACGATACCGGTGCCCCATCCCAAGAAGGATCTGCCCTTGGGCACCGTGCGAAGTATCC
GGCAGCGTGCCGGGTTGTTTTCGTTCTAG

Antitoxin

Protein

• Length: 135 a.a.
• Molecular weight: 14706.68 Da
• Isoelectric Point: 4.6115

>AT112004 WP_057438841.1 NZ_CP032618:1814962-1815366 [Pseudomonas fluorescens]
MQYPICIEWGDDFTATGIQIPDIPGAVTAGDSFEEAYNAAVEVAHILLQELAGEGRAIPMPTSVATHHGNEEYAGMGWGM
LELDISPYLGKTEKVNVTLPGYVIQRIDRYVREHKVKSRSSFLADAALEKLVRS

Nucleotide

• Length: 405 bp

>AT112004 NZ_CP032618:1814962-1815366 [Pseudomonas fluorescens]
ATGCAATACCCAATCTGTATCGAATGGGGCGATGACTTTACCGCCACCGGTATCCAGATCCCTGATATTCCAGGCGCGGT
GACGGCCGGGGACAGTTTTGAAGAGGCTTACAACGCCGCCGTCGAAGTGGCTCACATCCTGTTGCAGGAGCTGGCTGGCG
AAGGCCGCGCGATTCCGATGCCGACCTCGGTGGCGACGCACCACGGCAATGAGGAGTACGCCGGGATGGGCTGGGGGATG
CTCGAGCTCGATATCTCGCCTTACCTGGGCAAGACCGAGAAGGTCAATGTCACGTTGCCGGGGTATGTGATCCAGCGTAT
TGACCGTTATGTGCGCGAGCACAAGGTCAAAAGTCGCTCGTCATTCCTGGCGGATGCGGCGCTGGAGAAGTTGGTGCGTT
CTTGA