Detailed information of TA system TA112002

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 1302892..1303758
• Replicon: chromosome
• Accession: NZ_CP032618
• Organism: Pseudomonas fluorescens strain PF08

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: K0WRS8
• Locus tag: D7M10_RS05905
• Protein ID: WP_003229979.1
• Coordinates: 1303579..1303758 (-)
• Length: 60 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: D7M10_RS05900
• Protein ID: WP_120731860.1
• Coordinates: 1302892..1303527 (-)
• Length: 212 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D7M10_RS05865	1298320..1299270	+	951	WP_120731846.1	hypothetical protein	-
D7M10_RS05870	1299251..1300033	+	783	WP_120731848.1	ATP-binding protein	-
D7M10_RS05875	1300033..1300362	+	330	WP_120731850.1	hypothetical protein	-
D7M10_RS05880	1300355..1300942	+	588	WP_120731852.1	DUF1367 family protein	-
D7M10_RS05885	1300939..1301613	+	675	WP_120731854.1	recombination protein NinG	-
D7M10_RS05890	1301610..1302287	+	678	WP_120731856.1	hypothetical protein	-
D7M10_RS05895	1302659..1302859	-	201	WP_120731857.1	hypothetical protein	-
D7M10_RS05900	1302892..1303527	-	636	WP_120731860.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
D7M10_RS05905	1303579..1303758	-	180	WP_003229979.1	type II toxin-antitoxin system HicA family toxin	Toxin
D7M10_RS05910	1303846..1304217	+	372	WP_105523600.1	chemotaxis protein	-
D7M10_RS05915	1304217..1304534	+	318	WP_057959962.1	hypothetical protein	-
D7M10_RS05920	1304588..1304803	+	216	WP_120731862.1	hypothetical protein	-
D7M10_RS05925	1304800..1304991	+	192	WP_120731864.1	hypothetical protein	-
D7M10_RS05930	1304988..1305242	+	255	WP_120731866.1	hypothetical protein	-
D7M10_RS05935	1305274..1305483	-	210	WP_120731868.1	hypothetical protein	-
D7M10_RS05940	1305573..1306172	+	600	WP_120731870.1	hypothetical protein	-
D7M10_RS05945	1306174..1307664	+	1491	WP_120731872.1	terminase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1278859..1335045	56186

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (4-59)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 60 a.a.
• Molecular weight: 6632.67 Da
• Isoelectric Point: 11.1475

>T112002 WP_003229979.1 NZ_CP032618:c1303758-1303579 [Pseudomonas fluorescens]
MKFSEFRRWLKAQGVTFEAGKGSHFKVIAPNGNRSTFADHGSKEMPEPTRKAIIKQLGL

Nucleotide

• Length: 180 bp

>T112002 NZ_CP032618:c1303758-1303579 [Pseudomonas fluorescens]
ATGAAGTTCAGCGAGTTCAGACGATGGTTGAAGGCCCAAGGGGTGACCTTCGAAGCAGGCAAAGGAAGCCACTTCAAAGT
CATCGCCCCAAACGGCAACAGAAGCACCTTCGCGGATCACGGCAGTAAAGAAATGCCAGAACCGACCCGCAAGGCGATCA
TTAAACAACTGGGGCTCTGA

Antitoxin

Protein

• Length: 212 a.a.
• Molecular weight: 23646.78 Da
• Isoelectric Point: 5.1292

>AT112002 WP_120731860.1 NZ_CP032618:c1303527-1302892 [Pseudomonas fluorescens]
MYNYAIRFEQDSAPGLAVFCRDLPELNSYGDDKEHAIREAVDAIETALSIYVDERRAIPQASPAQPGEHVIRLPAVTVAK
IVLWNEMMAQDMRKADLCRLLGLAQTQGDRLVDFLHTSKMEAIEDALKPFGVGLSVSREALPSYIEYEVTYAQSGEVHSF
RFLENQHLTNEDAWGKIQARLKLVNRHLPGTPSSKETCESHGVTDVKITYA

Nucleotide

• Length: 636 bp

>AT112002 NZ_CP032618:c1303527-1302892 [Pseudomonas fluorescens]
ATGTACAACTACGCAATCCGCTTTGAGCAAGACAGCGCACCAGGGCTGGCCGTGTTCTGCCGCGACCTGCCAGAGCTGAA
CAGCTACGGCGACGACAAAGAACACGCCATCCGCGAAGCCGTGGATGCGATTGAGACAGCCCTGTCGATCTATGTGGACG
AGCGCCGAGCCATCCCTCAGGCATCACCAGCGCAGCCAGGCGAACACGTTATTCGTCTTCCGGCCGTAACCGTGGCCAAG
ATCGTTCTGTGGAACGAAATGATGGCGCAGGATATGCGCAAGGCTGATCTGTGCCGGCTTCTGGGGTTGGCTCAGACACA
AGGTGATCGTCTTGTCGACTTCCTCCACACATCGAAGATGGAAGCAATTGAGGACGCCCTGAAGCCATTTGGCGTGGGGC
TATCAGTGTCCAGAGAAGCTTTGCCTAGCTATATCGAGTACGAAGTGACCTATGCTCAGTCTGGCGAGGTTCATAGCTTC
CGCTTTCTGGAAAACCAACACCTCACCAATGAAGATGCGTGGGGAAAGATCCAGGCACGCTTAAAGCTGGTCAATCGGCA
TCTCCCTGGCACGCCCTCATCAAAAGAGACTTGCGAGTCTCACGGGGTAACCGACGTAAAAATCACTTACGCATAG

Structures

Toxin

• AlphaFold DB: A0A5R8R2U7