Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 2025900..2026542 Replicon chromosome
Accession NZ_CP032608
Organism Bacillus thuringiensis strain QZL38

Toxin (Protein)


Gene name pemK Uniprot ID R8I8B8
Locus tag D7J84_RS10270 Protein ID WP_000635965.1
Coordinates 2025900..2026250 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID R8I8H2
Locus tag D7J84_RS10275 Protein ID WP_000004570.1
Coordinates 2026255..2026542 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
D7J84_RS10255 2022836..2023294 - 459 WP_061883709.1 SprT family protein -
D7J84_RS10260 2023490..2023606 + 117 WP_001143642.1 cortex morphogenetic protein CmpA -
D7J84_RS10265 2023664..2025832 - 2169 WP_000426236.1 RNA-binding transcriptional accessory protein -
D7J84_RS10270 2025900..2026250 - 351 WP_000635965.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
D7J84_RS10275 2026255..2026542 - 288 WP_000004570.1 antitoxin EndoAI Antitoxin
D7J84_RS10280 2026851..2028020 - 1170 WP_000390613.1 alanine racemase -
D7J84_RS10285 2028138..2029088 - 951 WP_002004297.1 outer membrane lipoprotein carrier protein LolA -
D7J84_RS10290 2029245..2029604 - 360 WP_000583417.1 holo-ACP synthase -
D7J84_RS10295 2029698..2030270 + 573 WP_000908521.1 rhomboid family intramembrane serine protease -
D7J84_RS10300 2030263..2031225 - 963 WP_000961160.1 UV DNA damage repair endonuclease UvsE -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12948.04 Da        Isoelectric Point: 5.7168

>T111990 WP_000635965.1 NZ_CP032608:c2026250-2025900 [Bacillus thuringiensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDEVMMSRVDEALQISLGLIDF

Download         Length: 351 bp

>T111990 NZ_CP032608:c2026250-2025900 [Bacillus thuringiensis]
TTGATTGTAAAACGCGGCGACGTGTATTTTGCAGACCTTTCCCCAGTTGTTGGTTCTGAGCAAGGAGGCGTTCGTCCGGT
TCTTGTCATTCAAAATGACATCGGAAATCGTTTTAGTCCAACGGTGATTGTAGCGGCTATTACTGCACAGATTCAAAAAG
CGAAATTACCCACTCATGTGGAAATTGATGCGAAAAAGTATGGTTTTGAGAGAGATTCTGTTATTTTACTTGAGCAGATT
CGAACAATCGATAAGCAACGCTTAACGGACAAAATCACTCATTTGGATGAAGTGATGATGAGTCGTGTAGATGAAGCGTT
ACAAATTAGTTTAGGACTAATAGATTTTTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10780.36 Da        Isoelectric Point: 8.9605

>AT111990 WP_000004570.1 NZ_CP032608:c2026542-2026255 [Bacillus thuringiensis]
VSESSVTTEIVVRLPKQMVTELDGIGKQENKNRHELICQATQLLLRQHKTKKRYQHESMRRGYIEMGKINLGIASEAFLA
EYEAAHTVERLVSGG

Download         Length: 288 bp

>AT111990 NZ_CP032608:c2026542-2026255 [Bacillus thuringiensis]
GTGTCCGAATCAAGTGTAACTACTGAAATCGTGGTTCGGTTGCCAAAGCAAATGGTAACGGAATTGGACGGAATTGGAAA
ACAAGAGAATAAGAATCGCCATGAACTAATTTGCCAGGCAACACAATTGTTATTGCGTCAACATAAGACGAAGAAACGCT
ACCAACATGAATCAATGCGACGTGGGTACATTGAAATGGGAAAAATTAATCTTGGTATTGCATCTGAAGCTTTCTTAGCA
GAGTATGAAGCAGCTCATACAGTAGAACGCTTAGTTAGCGGGGGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A366G118

Antitoxin

Source ID Structure
AlphaFold DB A0A366FY90

References