Detailed information of TA system TA111986

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/COG3657-dnstrm_HI1420
• Location: 5089075..5089661
• Replicon: chromosome
• Accession: NZ_CP032569
• Organism: Pseudomonas aeruginosa strain BA7823

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: G8CP73
• Locus tag: D7H65_RS24215
• Protein ID: WP_003120987.1
• Coordinates: 5089362..5089661 (-)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: D7H65_RS24210
• Protein ID: WP_003448662.1
• Coordinates: 5089075..5089365 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D7H65_RS24195	5084656..5086545	+	1890	WP_023093670.1	hypothetical protein	-
D7H65_RS24200	5086542..5088518	+	1977	WP_023093671.1	DEAD/DEAH box helicase	-
D7H65_RS24205	5088657..5088992	+	336	WP_022579623.1	hypothetical protein	-
D7H65_RS24210	5089075..5089365	-	291	WP_003448662.1	putative addiction module antidote protein	Antitoxin
D7H65_RS24215	5089362..5089661	-	300	WP_003120987.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
D7H65_RS24220	5089863..5090987	+	1125	WP_024007699.1	TcpQ domain-containing protein	-
D7H65_RS24225	5090987..5092696	+	1710	WP_003123331.1	PilN family type IVB pilus formation outer membrane protein	-
D7H65_RS24230	5092700..5094025	+	1326	WP_022579625.1	type 4b pilus protein PilO2	-
D7H65_RS24235	5094015..5094548	+	534	WP_003149521.1	type IV pilus biogenesis protein PilP	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	5058369..5152012	93643

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (3-96)

Antitoxin

• Cro/C1-type helix-turn-helix domain (47-90)

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 11133.88 Da
• Isoelectric Point: 10.4495

>T111986 WP_003120987.1 NZ_CP032569:c5089661-5089362 [Pseudomonas aeruginosa]
MVEVKQTATFMAWESKLKDRRAKAVIAARIFRLANGLPGDVSPVGQGVSELRIHYGPGYRVYFQQRGTEIVILLCGGDKS
SQARDIEMAKRLANEWRPQ

Nucleotide

• Length: 300 bp

>T111986 NZ_CP032569:c5089661-5089362 [Pseudomonas aeruginosa]
ATGGTTGAAGTCAAACAAACCGCCACTTTCATGGCATGGGAAAGCAAGCTAAAGGATCGGCGGGCAAAGGCCGTGATCGC
CGCCCGTATCTTCCGATTGGCGAATGGACTACCCGGCGATGTGTCACCAGTCGGCCAGGGTGTGAGCGAGTTGAGGATTC
ACTATGGCCCAGGCTATCGGGTGTACTTCCAGCAGCGCGGGACGGAAATCGTCATCCTGCTATGTGGTGGAGACAAGAGC
AGCCAGGCCAGGGATATAGAAATGGCGAAACGCCTGGCAAATGAGTGGAGGCCGCAATGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10394.91 Da
• Isoelectric Point: 4.7527

>AT111986 WP_003448662.1 NZ_CP032569:c5089365-5089075 [Pseudomonas aeruginosa]
MTEQIYDYDPAQALDSPEAMALFIADALDTGDTAYIAKAMGVVARAKGMTELAKETGLAREQLYKSFSERGNPTLKTMLA
VMRALGVDLTARPHAQ

Nucleotide

• Length: 291 bp

>AT111986 NZ_CP032569:c5089365-5089075 [Pseudomonas aeruginosa]
ATGACCGAGCAAATTTATGATTATGACCCCGCGCAAGCGCTGGACAGCCCGGAGGCCATGGCTCTGTTCATCGCAGACGC
CTTGGATACTGGAGACACCGCCTATATCGCTAAGGCTATGGGAGTCGTGGCTCGCGCCAAGGGGATGACGGAGTTGGCCA
AGGAAACGGGTTTGGCCAGGGAGCAGCTTTACAAATCGTTTAGTGAGCGCGGGAACCCGACATTGAAAACCATGCTGGCA
GTAATGCGAGCTTTGGGCGTTGATCTGACCGCCCGCCCGCATGCTCAGTGA

Structures

Toxin

• AlphaFold DB: G8CP73