Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) PfiT-PfiA/ParE(toxin)
Location 4686459..4687067 Replicon chromosome
Accession NZ_CP032569
Organism Pseudomonas aeruginosa strain BA7823

Toxin (Protein)


Gene name PfiT Uniprot ID A0A444LUU5
Locus tag D7H65_RS22290 Protein ID WP_019486378.1
Coordinates 4686459..4686806 (-) Length 116 a.a.

Antitoxin (Protein)


Gene name PfiA Uniprot ID A0A0B0C355
Locus tag D7H65_RS22295 Protein ID WP_003114155.1
Coordinates 4686816..4687067 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
D7H65_RS22260 4681818..4682090 + 273 WP_074241374.1 cysteine-rich CWC family protein -
D7H65_RS22265 4682090..4682782 + 693 WP_003085458.1 16S rRNA pseudouridine(516) synthase -
D7H65_RS22270 4682918..4683961 + 1044 WP_003134392.1 L,D-transpeptidase -
D7H65_RS22275 4684041..4684778 + 738 WP_003085453.1 murein L,D-transpeptidase catalytic domain family protein -
D7H65_RS22280 4685230..4686132 + 903 WP_003085447.1 (R)-3-hydroxydecanoyl-ACP:CoA transacylase -
D7H65_RS22290 4686459..4686806 - 348 WP_019486378.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
D7H65_RS22295 4686816..4687067 - 252 WP_003114155.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
D7H65_RS22300 4687281..4688264 - 984 WP_033990852.1 tyrosine-type recombinase/integrase -
D7H65_RS22305 4688264..4689556 - 1293 WP_033990854.1 hypothetical protein -
D7H65_RS22310 4689786..4691060 - 1275 WP_033990855.1 zonular occludens toxin family protein -
D7H65_RS22315 4691064..4691420 - 357 WP_003114150.1 DUF2523 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage catB7 - 4686459..4709551 23092


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(6-100)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 116 a.a.        Molecular weight: 12956.72 Da        Isoelectric Point: 4.4212

>T111985 WP_019486378.1 NZ_CP032569:c4686806-4686459 [Pseudomonas aeruginosa]
MSPVVIRFTDTAEQSIEDQVHHLAPFQGEQAALQSVLSLLDEIEEKISLAPKGYPVSQQASLLGVLSYRELNTGPYRVFY
EFHEEQGEAAVILVLRQKQSVEQQLIRYCLVGPIE

Download         Length: 348 bp

>T111985 NZ_CP032569:c4686806-4686459 [Pseudomonas aeruginosa]
ATGTCCCCGGTCGTCATTCGTTTTACTGATACCGCAGAGCAAAGCATCGAAGACCAAGTCCACCACTTGGCTCCATTCCA
AGGTGAACAGGCTGCACTCCAGTCAGTACTGAGCCTTTTGGATGAGATTGAAGAGAAGATTTCACTTGCACCTAAAGGTT
ACCCAGTCAGCCAGCAGGCGAGTCTTCTGGGGGTGCTGAGCTATCGCGAGCTTAATACCGGCCCCTATCGTGTTTTTTAC
GAATTCCACGAAGAGCAAGGCGAGGCGGCAGTGATCTTGGTTTTGCGACAGAAGCAGAGCGTTGAGCAGCAATTGATCCG
CTACTGCTTGGTGGGGCCAATCGAGTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9442.79 Da        Isoelectric Point: 9.4808

>AT111985 WP_003114155.1 NZ_CP032569:c4687067-4686816 [Pseudomonas aeruginosa]
MRVETISYLKRHAADLDLSEPMVVTQNGVPAYVVESYAERKQRDEAIALVKLLAIGSRQYAEGKHRSVDDLKARLSRRFA
QPE

Download         Length: 252 bp

>AT111985 NZ_CP032569:c4687067-4686816 [Pseudomonas aeruginosa]
ATGCGAGTCGAGACAATTAGTTATTTGAAACGTCATGCGGCTGACCTGGATTTATCCGAGCCAATGGTCGTCACGCAGAA
CGGTGTTCCTGCCTATGTGGTTGAGTCATATGCTGAGCGGAAGCAGCGCGATGAAGCAATTGCGCTGGTGAAGTTGCTTG
CGATTGGCTCCCGCCAGTACGCAGAAGGCAAGCATCGCTCTGTTGATGATTTGAAAGCTCGCCTTTCCAGGAGGTTCGCT
CAGCCAGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A444LUU5

Antitoxin

Source ID Structure
AlphaFold DB A0A0B0C355

References