Detailed information of TA system TA111980

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/ParE-CC2985
• Location: 21291..21825
• Replicon: plasmid pIB1
• Accession: NZ_CP032567
• Organism: Yersinia pseudotuberculosis strain IP2666pIB1

Toxin (Protein)

• Gene name: parE
• Uniprot ID: Q663N8
• Locus tag: DN756_RS21695
• Protein ID: WP_002213360.1
• Coordinates: 21291..21590 (-)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: Q9ZGW3
• Locus tag: DN756_RS21700
• Protein ID: WP_002224342.1
• Coordinates: 21583..21825 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DN756_RS21665	16723..17148	-	426	WP_002213267.1	type III secretion system chaperone SycH	-
DN756_RS21670	17526..17924	-	399	Protein_22	ATP-binding protein	-
DN756_RS21675	17924..19096	-	1173	WP_011191364.1	IS21 family transposase	-
DN756_RS21680	19199..19843	+	645	Protein_24	IS3 family transposase	-
DN756_RS21685	19906..20298	-	393	WP_002213403.1	type III secretion system chaperone SycE/YerA	-
DN756_RS21690	20492..21151	+	660	WP_123767105.1	type III secretion system effector GTPase activator YopE	-
DN756_RS21695	21291..21590	-	300	WP_002213360.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
DN756_RS21700	21583..21825	-	243	WP_002224342.1	type II toxin-antitoxin system ParD family antitoxin	Antitoxin
DN756_RS21705	21991..22283	-	293	Protein_29	IS6 family transposase	-
DN756_RS22200	22368..22541	+	174	WP_002213357.1	hypothetical protein	-
DN756_RS22205	22690..22971	-	282	WP_164491551.1	hypothetical protein	-
DN756_RS21715	23131..24096	-	966	WP_002213354.1	ParB/RepB/Spo0J family plasmid partition protein	-
DN756_RS21720	24093..25259	-	1167	WP_002220902.1	plasmid-partitioning protein SopA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	yopO/ypkA / yadA / sycH / sycE/yerA / yopE / yopK/yopQ / yopT / yopM / yopD / yopB / sycD/lcrH / lcrV / lcrG / lcrR / yscV/lcrD / yscY / yscX / sycN / tyeA / lcrE/yopN / yscN / yscO / yscP / yscQ / yscR / yscS / yscT / yscU / virG/yscW / yscB / yscC / yscD / yscE / yscF / yscG / yopR/yscH/lcrP / lcrO/yscI / ylpB/yscJ / yscK / yscL / lcrQ/yscM / yopH / yopJ/yopP	1..66625	66625

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (2-89)

Antitoxin

• Antitoxin ParD (5-72)

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 11632.19 Da
• Isoelectric Point: 5.5114

>T111980 WP_002213360.1 NZ_CP032567:c21590-21291 [Yersinia pseudotuberculosis]
VYKLSELADEDIYNIASYTIRHFGVTQAKLYHENLAKVFELLAKNPELGAECNWICSDIRRFQYKKHGIYYITLSNDILI
SRVLHQSIDIDVQDFPEHE

Nucleotide

• Length: 300 bp

>T111980 NZ_CP032567:c21590-21291 [Yersinia pseudotuberculosis]
GTGTATAAACTGTCTGAACTAGCTGATGAGGATATTTATAATATTGCCAGTTATACTATCCGGCATTTCGGTGTGACTCA
GGCTAAGTTATACCATGAGAACTTGGCAAAGGTATTCGAGCTATTAGCTAAAAATCCAGAGTTAGGGGCTGAGTGTAACT
GGATTTGCTCTGATATTCGCCGTTTTCAGTATAAAAAGCACGGTATATACTATATAACGCTTAGCAATGATATTTTGATT
TCTCGAGTGCTGCATCAATCCATAGATATAGATGTTCAGGATTTTCCAGAGCATGAGTAG

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 8865.15 Da
• Isoelectric Point: 6.4781

>AT111980 WP_002224342.1 NZ_CP032567:c21825-21583 [Yersinia pseudotuberculosis]
MAHVTSVTLGEHLTGFVGEMIQSGRYGNISEVLRDALRLMEAREQRVQHVRDMVLAGTNVPVSHRLMDEIFSAAVKDTSV

Nucleotide

• Length: 243 bp

>AT111980 NZ_CP032567:c21825-21583 [Yersinia pseudotuberculosis]
ATGGCTCACGTAACCAGCGTAACACTTGGAGAGCATTTGACAGGTTTTGTGGGGGAAATGATTCAGTCTGGTCGTTATGG
CAATATATCAGAAGTGCTTCGTGATGCCTTAAGGCTAATGGAAGCACGTGAACAGCGTGTTCAACATGTACGCGATATGG
TGCTTGCAGGAACAAATGTACCTGTGAGCCATCGTTTAATGGATGAGATTTTTTCTGCTGCGGTGAAAGATACTAGTGTA
TAA

Structures

Toxin

• AlphaFold DB: Q663N8

Antitoxin

• AlphaFold DB: A0A0E1NQY6