Detailed information of TA system TA111979

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Hha-TomB/-
• Location: 3439883..3440503
• Replicon: chromosome
• Accession: NZ_CP032566
• Organism: Yersinia pseudotuberculosis strain IP2666pIB1

Toxin (Protein)

• Gene name: Hha
• Uniprot ID: Q66DR5
• Locus tag: DN756_RS16010
• Protein ID: WP_002208622.1
• Coordinates: 3440300..3440503 (+)
• Length: 68 a.a.

Antitoxin (Protein)

• Gene name: TomB
• Uniprot ID: Q66DR4
• Locus tag: DN756_RS16005
• Protein ID: WP_002218472.1
• Coordinates: 3439883..3440251 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DN756_RS15985	3437392..3437850	+	459	WP_002213775.1	IS200/IS605-like element IS1541B family transposase	-
DN756_RS15990	3438088..3438348	+	261	WP_002208617.1	type B 50S ribosomal protein L31	-
DN756_RS15995	3438364..3438507	+	144	WP_002208618.1	type B 50S ribosomal protein L36	-
DN756_RS16000	3439371..3439724	+	354	WP_012105569.1	hypothetical protein	-
DN756_RS16005	3439883..3440251	+	369	WP_002218472.1	Hha toxicity modulator TomB	Antitoxin
DN756_RS16010	3440300..3440503	+	204	WP_002208622.1	expression modulating protein YmoA	Toxin
DN756_RS16020	3441409..3441798	+	390	WP_024063215.1	MGMT family protein	-
DN756_RS16025	3441934..3442455	-	522	WP_011191872.1	YbaY family lipoprotein	-
DN756_RS16030	3442712..3443572	+	861	WP_002208625.1	acyl-CoA thioesterase II	-
DN756_RS16035	3443833..3445122	-	1290	WP_002228344.1	ammonium transporter AmtB	-
DN756_RS16040	3445162..3445500	-	339	WP_002208627.1	P-II family nitrogen regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	3437392..3437850	458

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (8-64)

Antitoxin

• Biofilm formation regulator YbaJ (1-118)

Sequences

Toxin

Protein

• Length: 68 a.a.
• Molecular weight: 8063.31 Da
• Isoelectric Point: 6.4573

>T111979 WP_002208622.1 NZ_CP032566:3440300-3440503 [Yersinia pseudotuberculosis]
MTKTDYLMRLRKCTTIDTLERVIEKNKYELSDDELELFYSAADHRLAELTMNKLYDKIPPTVWQHVK

Nucleotide

• Length: 204 bp

>T111979 NZ_CP032566:3440300-3440503 [Yersinia pseudotuberculosis]
ATGACAAAAACTGACTACCTGATGCGTTTAAGAAAATGTACGACTATCGATACGCTAGAACGTGTAATTGAAAAAAATAA
GTACGAACTTTCTGATGATGAACTGGAATTGTTTTACTCAGCTGCAGACCATCGCCTGGCTGAACTAACTATGAATAAGC
TTTATGATAAAATTCCACCTACTGTATGGCAACATGTGAAATAG

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 14215.00 Da
• Isoelectric Point: 4.6121

>AT111979 WP_002218472.1 NZ_CP032566:3439883-3440251 [Yersinia pseudotuberculosis]
MDEYSPKRHDIAQLKFLCESLYDEGIATLGDSHHGWINDPTSAINLQLNDLIEHIASFVMSFKIKYPDGSQLSELVEEYL
DDTYTLFSSYGINDPELRRWQKTKEHLFRLFSGEYVCTLMKT

Nucleotide

• Length: 369 bp

>AT111979 NZ_CP032566:3439883-3440251 [Yersinia pseudotuberculosis]
ATGGATGAGTACTCGCCTAAGCGGCATGACATTGCCCAACTGAAGTTTCTATGTGAAAGCTTGTATGATGAAGGTATTGC
AACTCTTGGCGACAGCCATCATGGCTGGATAAATGACCCAACGTCGGCCATAAACCTTCAACTCAATGATTTGATCGAGC
ATATAGCTTCATTTGTGATGAGTTTTAAAATAAAATACCCGGATGGAAGCCAGCTTTCCGAGTTAGTGGAAGAATATTTA
GACGACACATATACTCTGTTTAGTAGTTATGGAATCAATGATCCTGAATTGCGTCGCTGGCAGAAAACCAAAGAGCATTT
ATTCAGACTTTTCTCAGGGGAATACGTATGTACCCTGATGAAAACTTAA

Structures

Toxin

• PDB: 2K5S

Antitoxin

• AlphaFold DB: Q66DR4