Detailed information of TA system TA111966

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/COG3657-dnstrm_HI1420
• Location: 5488043..5488629
• Replicon: chromosome
• Accession: NZ_CP032552
• Organism: Pseudomonas aeruginosa strain PA34

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: G8CP73
• Locus tag: PA34_RS26375
• Protein ID: WP_003120987.1
• Coordinates: 5488330..5488629 (-)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: PA34_RS26370
• Protein ID: WP_003448662.1
• Coordinates: 5488043..5488333 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PA34_RS26355	5483624..5485513	+	1890	WP_033945085.1	hypothetical protein	-
PA34_RS26360	5485510..5487486	+	1977	WP_114249722.1	DEAD/DEAH box helicase	-
PA34_RS26365	5487625..5487960	+	336	WP_022579623.1	hypothetical protein	-
PA34_RS26370	5488043..5488333	-	291	WP_003448662.1	putative addiction module antidote protein	Antitoxin
PA34_RS26375	5488330..5488629	-	300	WP_003120987.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
PA34_RS26380	5488831..5489943	+	1113	WP_114249721.1	TcpQ domain-containing protein	-
PA34_RS26385	5489943..5491652	+	1710	WP_114249720.1	PilN family type IVB pilus formation outer membrane protein	-
PA34_RS26390	5491656..5492981	+	1326	WP_033942384.1	type 4b pilus protein PilO2	-
PA34_RS26395	5492971..5493504	+	534	WP_023094340.1	type IV pilus biogenesis protein PilP	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	5459604..5545649	86045

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (3-96)

Antitoxin

• Cro/C1-type helix-turn-helix domain (47-90)

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 11133.88 Da
• Isoelectric Point: 10.4495

>T111966 WP_003120987.1 NZ_CP032552:c5488629-5488330 [Pseudomonas aeruginosa]
MVEVKQTATFMAWESKLKDRRAKAVIAARIFRLANGLPGDVSPVGQGVSELRIHYGPGYRVYFQQRGTEIVILLCGGDKS
SQARDIEMAKRLANEWRPQ

Nucleotide

• Length: 300 bp

>T111966 NZ_CP032552:c5488629-5488330 [Pseudomonas aeruginosa]
ATGGTTGAAGTCAAACAAACCGCCACTTTCATGGCATGGGAAAGCAAGCTAAAGGATCGGCGGGCAAAGGCCGTGATCGC
CGCCCGTATCTTCCGATTGGCGAATGGACTACCCGGCGATGTGTCACCAGTCGGCCAGGGTGTGAGCGAGTTGAGGATTC
ACTATGGCCCAGGCTATCGGGTGTACTTCCAGCAGCGCGGGACGGAAATCGTCATCCTGCTATGTGGTGGAGACAAGAGC
AGCCAGGCCAGGGATATAGAAATGGCGAAACGCCTGGCAAATGAGTGGAGGCCGCAATGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10394.91 Da
• Isoelectric Point: 4.7527

>AT111966 WP_003448662.1 NZ_CP032552:c5488333-5488043 [Pseudomonas aeruginosa]
MTEQIYDYDPAQALDSPEAMALFIADALDTGDTAYIAKAMGVVARAKGMTELAKETGLAREQLYKSFSERGNPTLKTMLA
VMRALGVDLTARPHAQ

Nucleotide

• Length: 291 bp

>AT111966 NZ_CP032552:c5488333-5488043 [Pseudomonas aeruginosa]
ATGACCGAGCAAATTTATGATTATGACCCCGCGCAAGCGCTGGACAGCCCGGAGGCCATGGCTCTGTTCATCGCAGACGC
CTTGGATACTGGAGACACCGCCTATATCGCTAAGGCTATGGGAGTCGTGGCTCGCGCCAAGGGGATGACGGAGTTGGCCA
AGGAAACGGGTTTGGCCAGGGAGCAGCTTTACAAATCGTTTAGTGAGCGCGGGAACCCGACATTGAAAACCATGCTGGCA
GTAATGCGAGCTTTGGGCGTTGATCTGACCGCCCGCCCGCATGCTCAGTGA

Structures

Toxin

• AlphaFold DB: G8CP73