Detailed information of TA system TA111965

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 5477830..5478482
• Replicon: chromosome
• Accession: NZ_CP032552
• Organism: Pseudomonas aeruginosa strain PA34

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: PA34_RS26325
• Protein ID: WP_033945041.1
• Coordinates: 5477830..5478240 (-)
• Length: 137 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: PA34_RS26330
• Protein ID: WP_168928567.1
• Coordinates: 5478240..5478482 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PA34_RS32940	5474148..5474828	+	681	WP_154112681.1	hypothetical protein	-
PA34_RS26290	5474841..5475569	+	729	WP_031275718.1	TIGR03761 family integrating conjugative element protein	-
PA34_RS26295	5475575..5476108	+	534	WP_003134105.1	DUF3158 family protein	-
PA34_RS26300	5476122..5476610	+	489	WP_003134107.1	single-stranded DNA-binding protein	-
PA34_RS26305	5476714..5476908	+	195	WP_033945044.1	hypothetical protein	-
PA34_RS26310	5477152..5477319	+	168	WP_128729268.1	CrpP family protein	-
PA34_RS26315	5477385..5477603	-	219	WP_033945042.1	hypothetical protein	-
PA34_RS26320	5477620..5477814	-	195	WP_023094845.1	hypothetical protein	-
PA34_RS26325	5477830..5478240	-	411	WP_033945041.1	tRNA(fMet)-specific endonuclease VapC	Toxin
PA34_RS26330	5478240..5478482	-	243	WP_168928567.1	antitoxin	Antitoxin
PA34_RS26335	5478788..5480707	+	1920	WP_033945040.1	type I DNA topoisomerase	-
PA34_RS26340	5480715..5481467	+	753	WP_033945033.1	hypothetical protein	-
PA34_RS26345	5481584..5482189	-	606	WP_033945032.1	LysE family translocator	-
PA34_RS26350	5482311..5483180	+	870	WP_023094849.1	LysR family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	5459604..5545649	86045

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-124)

Antitoxin

• SpoVT-AbrB domain (11-37)

Sequences

Toxin

Protein

• Length: 137 a.a.
• Molecular weight: 15452.83 Da
• Isoelectric Point: 7.9073

>T111965 WP_033945041.1 NZ_CP032552:c5478240-5477830 [Pseudomonas aeruginosa]
MLKFMLDTNICIFTIKKRPQEVREAFLRHHDQMCISTVTLMELIYGAEKSSNPSRNLADVEGFAARLEVLKYDQDAAAHT
GQLRAELARLGKQIGPYDQMIAGHARSQGLIVVTNNRREFDRVPGLRVEDWVTPIV

Nucleotide

• Length: 411 bp

>T111965 NZ_CP032552:c5478240-5477830 [Pseudomonas aeruginosa]
ATGCTCAAGTTCATGCTTGACACCAACATCTGCATCTTCACCATCAAAAAACGTCCGCAAGAGGTGCGCGAGGCGTTCCT
GCGCCATCACGACCAGATGTGCATCAGCACCGTGACGCTGATGGAGCTGATCTACGGCGCAGAGAAGTCCTCGAACCCCT
CGCGCAATCTTGCAGACGTGGAAGGCTTCGCTGCTCGTCTGGAGGTGCTCAAGTACGACCAGGACGCTGCCGCCCACACC
GGCCAGCTCCGCGCTGAACTGGCCCGCCTCGGCAAGCAAATCGGCCCCTATGACCAGATGATCGCCGGGCACGCCCGCTC
TCAGGGCCTGATCGTGGTCACCAATAACCGCCGCGAGTTCGACCGCGTACCCGGCTTGAGAGTTGAGGATTGGGTCACCC
CAATCGTATAG

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 8923.97 Da
• Isoelectric Point: 4.4230

>AT111965 WP_168928567.1 NZ_CP032552:c5478482-5478240 [Pseudomonas aeruginosa]
MRCIVEQGSVFQSNRSQAVRLPKAVALPDDVKRVDVVAVGRARIITPAGESWDSWFDGEGVTADFMAEREQPADQERETF

Nucleotide

• Length: 243 bp

>AT111965 NZ_CP032552:c5478482-5478240 [Pseudomonas aeruginosa]
ATGAGGTGTATCGTGGAACAAGGTTCTGTTTTTCAGAGCAATCGTAGCCAGGCCGTAAGACTGCCCAAGGCCGTCGCGCT
TCCTGATGACGTTAAGCGTGTGGATGTGGTCGCTGTGGGGCGCGCCAGAATCATCACGCCTGCCGGTGAGTCTTGGGATA
GCTGGTTCGATGGCGAGGGCGTTACTGCCGACTTTATGGCAGAGCGTGAACAGCCTGCCGATCAGGAGCGCGAGACTTTC
TGA