Detailed information of TA system TA111964

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /Xre(antitoxin)
• Location: 4670778..4672036
• Replicon: chromosome
• Accession: NZ_CP032552
• Organism: Pseudomonas aeruginosa strain PA34

Toxin (Protein)

• Gene name: -
• Uniprot ID: V6AJ34
• Locus tag: PA34_RS22340
• Protein ID: WP_003086331.1
• Coordinates: 4670778..4671533 (-)
• Length: 252 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: V6AJU7
• Locus tag: PA34_RS22345
• Protein ID: WP_003086329.1
• Coordinates: 4671668..4672036 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PA34_RS22330	4665946..4668489	+	2544	WP_019486194.1	acyl-homoserine-lactone acylase QuiP	-
PA34_RS22335	4668644..4670005	-	1362	WP_003134034.1	DNA recombination protein RmuC	-
PA34_RS22340	4670778..4671533	-	756	WP_003086331.1	RES family NAD+ phosphorylase	Toxin
PA34_RS22345	4671668..4672036	-	369	WP_003086329.1	DUF2384 domain-containing protein	Antitoxin
PA34_RS22350	4672109..4673395	-	1287	WP_003086327.1	FAD-binding oxidoreductase	-
PA34_RS22355	4673451..4675040	-	1590	WP_003123191.1	aldehyde dehydrogenase family protein	-
PA34_RS22360	4675118..4675594	+	477	WP_003086322.1	hypothetical protein	-
PA34_RS33055	4675597..4675872	-	276	WP_023088486.1	zinc ribbon domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (63-207)

Antitoxin

• Cro/C1-type helix-turn-helix domain (23-47)

Sequences

Toxin

Protein

• Length: 252 a.a.
• Molecular weight: 28236.34 Da
• Isoelectric Point: 7.7294

>T111964 WP_003086331.1 NZ_CP032552:c4671533-4670778 [Pseudomonas aeruginosa]
VSEIWRQCKGERHVRPLQGRLVRLVESQEQVATLQLVDTLEEQALLEELLESSKPPVPADAEPLHYLLKTPFRYPPLRWG
SRFGRRHEPSLFYAALKLETAMAESAYYRCVLWSGMAVPPPSGRILSEHASFEAGWKVERGIRLQAPPFSDHEAALTDIA
DYRAPQELGSAMRSAGVQAFEYRSARCPERGCNVALFTPAAFTEKRPRNLTPWLCETTAGYVAFKPAHVPGSPKIFSWEL
FLVDGKLPHPA

Nucleotide

• Length: 756 bp

>T111964 NZ_CP032552:c4671533-4670778 [Pseudomonas aeruginosa]
GTGAGCGAGATCTGGCGACAGTGCAAGGGTGAGCGGCATGTCCGACCGCTCCAGGGGCGGCTGGTGCGGTTGGTGGAGAG
CCAGGAGCAGGTGGCGACCCTGCAATTGGTCGACACCCTGGAAGAACAGGCGTTGCTCGAGGAACTGCTCGAGTCGAGCA
AGCCGCCGGTGCCGGCGGATGCCGAGCCATTGCATTACCTGTTGAAGACCCCGTTCCGTTATCCGCCCTTGCGTTGGGGG
TCGCGGTTTGGCCGTCGTCACGAGCCAAGCCTGTTCTACGCCGCGTTGAAGCTGGAAACGGCCATGGCCGAAAGCGCGTA
TTACCGTTGTGTCCTGTGGAGTGGCATGGCGGTGCCGCCACCGAGCGGGAGGATTCTGTCCGAGCACGCTTCGTTCGAGG
CGGGCTGGAAGGTGGAGCGGGGAATCCGTCTGCAGGCGCCGCCTTTCTCCGATCACGAGGCTGCGCTGACCGACATCGCC
GACTACCGCGCTCCCCAGGAACTGGGGAGTGCCATGCGCAGCGCCGGGGTGCAGGCATTCGAATACCGTTCGGCGCGTTG
CCCGGAGCGCGGCTGCAATGTCGCCCTGTTCACGCCTGCGGCCTTCACCGAAAAGCGTCCTCGCAACCTGACGCCCTGGC
TGTGCGAAACCACGGCCGGTTACGTGGCCTTCAAGCCGGCGCATGTGCCGGGGTCGCCGAAAATCTTCTCCTGGGAGCTG
TTCCTGGTGGACGGAAAGTTGCCGCATCCGGCGTGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13367.63 Da
• Isoelectric Point: 9.9874

>AT111964 WP_003086329.1 NZ_CP032552:c4672036-4671668 [Pseudomonas aeruginosa]
MSALIKERPSADAVLAKAVLAAREQLGLTQLELAGIVGVDRSAISRWKTQGLRVDSKTGELALLLVRVYRALYALFGGQQ
EDMRHFLRTPNHHLAGEPLALMGQVQGLVHVLEYLDAIRGKV

Nucleotide

• Length: 369 bp

>AT111964 NZ_CP032552:c4672036-4671668 [Pseudomonas aeruginosa]
ATGAGCGCTCTCATCAAGGAACGTCCCAGCGCCGATGCTGTCCTGGCCAAGGCCGTCCTGGCCGCGCGCGAGCAATTGGG
GCTGACGCAGCTCGAACTGGCCGGCATCGTCGGCGTCGATCGCAGCGCCATCAGCCGCTGGAAGACCCAGGGCCTGCGGG
TGGACAGCAAGACCGGCGAGCTGGCTCTGCTGCTGGTGCGGGTCTATCGCGCACTGTATGCCCTGTTCGGCGGGCAGCAG
GAGGACATGCGCCACTTCCTGCGCACTCCCAACCATCACCTGGCGGGCGAGCCGCTGGCACTGATGGGACAGGTGCAGGG
CCTGGTTCATGTGCTGGAATACCTCGATGCGATCCGCGGCAAGGTCTGA

Structures

Toxin

• AlphaFold DB: V6AJ34

Antitoxin

• AlphaFold DB: V6AJU7