Detailed information of TA system TA111959

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relE-yefM/Txe-RelB
• Location: 245200..245729
• Replicon: chromosome
• Accession: NZ_CP032542
• Organism: Coxiella burnetii strain 2014-PE-15890

Toxin (Protein)

• Gene name: relE
• Uniprot ID: Q83AB3
• Locus tag: D6219_RS01460
• Protein ID: WP_005769718.1
• Coordinates: 245454..245729 (+)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: Q83AB4
• Locus tag: D6219_RS01455
• Protein ID: WP_005769716.1
• Coordinates: 245200..245454 (+)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D6219_RS01410	240302..241078	+	777	WP_017253343.1	16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA	-
D6219_RS01415	241085..241168	+	84	Protein_255	hypothetical protein	-
D6219_RS01420	241191..241625	+	435	WP_010958580.1	universal stress protein	-
D6219_RS01425	241794..242168	+	375	WP_005769706.1	hypothetical protein	-
D6219_RS01430	242213..242911	+	699	WP_010958581.1	outer membrane beta-barrel protein	-
D6219_RS01435	242975..243916	+	942	WP_005769709.1	S49 family peptidase	-
D6219_RS01440	243929..244804	+	876	WP_017253342.1	symmetrical bis(5'-nucleosyl)-tetraphosphatase	-
D6219_RS01445	244780..244893	+	114	Protein_261	type II toxin-antitoxin system YoeB family toxin	-
D6219_RS01450	244953..245087	+	135	WP_010958583.1	hypothetical protein	-
D6219_RS01455	245200..245454	+	255	WP_005769716.1	type II toxin-antitoxin system prevent-host-death family antitoxin	Antitoxin
D6219_RS01460	245454..245729	+	276	WP_005769718.1	Txe/YoeB family addiction module toxin	Toxin
D6219_RS01465	245702..246187	-	486	WP_005769725.1	dihydrofolate reductase	-
D6219_RS01470	246184..246987	-	804	WP_017253341.1	epoxyqueuosine reductase QueH	-
D6219_RS01475	247183..247470	+	288	WP_005769730.1	acylphosphatase	-
D6219_RS01480	247446..248000	-	555	WP_005769732.1	D-glycero-beta-D-manno-heptose 1,7-bisphosphate 7-phosphatase	-
D6219_RS01485	248013..248957	-	945	WP_010958586.1	methionyl-tRNA formyltransferase	-
D6219_RS01490	249124..249396	+	273	WP_129545620.1	hypothetical protein	-
D6219_RS01495	249393..250412	-	1020	WP_040953503.1	IS110-like element IS1111A family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	249393..250412	1019

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (5-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-74)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 11150.86 Da
• Isoelectric Point: 9.6762

>T111959 WP_005769718.1 NZ_CP032542:245454-245729 [Coxiella burnetii]
MQISFTPEAWEDYLYWQKFDKKMLRRINELIKDAMHEPFSGKGKPEPLKFELQGYWSRRLDQEHRLVYKVLDDSLMIIAA
RFHYNRLNSKN

Nucleotide

• Length: 276 bp

>T111959 NZ_CP032542:245454-245729 [Coxiella burnetii]
ATGCAAATTTCCTTCACGCCCGAAGCCTGGGAAGATTATTTATATTGGCAAAAATTCGACAAAAAAATGCTTCGACGCAT
TAATGAACTCATTAAGGATGCTATGCACGAGCCTTTTTCCGGAAAGGGAAAGCCAGAACCTTTAAAATTTGAATTACAAG
GATATTGGTCAAGACGATTAGATCAAGAACATCGATTGGTCTACAAAGTTTTAGACGATTCGTTAATGATTATCGCCGCA
AGATTTCACTATAATCGCCTTAATTCTAAAAACTGA

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9624.25 Da
• Isoelectric Point: 10.4802

>AT111959 WP_005769716.1 NZ_CP032542:245200-245454 [Coxiella burnetii]
MNVVTFSELRAQLKKILDLSADQHEPVVVKRPNKETMVILSLRDFEALKETAYLLSNEANAARLRQSIRSLKQGKAQKKK
LMED

Nucleotide

• Length: 255 bp

>AT111959 NZ_CP032542:245200-245454 [Coxiella burnetii]
ATGAACGTAGTTACTTTTAGCGAACTCAGGGCGCAACTTAAAAAAATCTTGGATCTTTCCGCCGATCAACACGAACCTGT
CGTCGTTAAACGGCCAAATAAAGAAACCATGGTCATTTTATCTTTACGCGACTTTGAGGCTCTAAAAGAAACAGCTTATC
TCTTAAGTAACGAAGCTAATGCGGCCCGTCTTCGTCAGTCTATCCGCAGCTTAAAACAAGGCAAGGCACAAAAAAAGAAA
TTAATGGAAGATTAA

Structures

Toxin

• AlphaFold DB: Q83AB3

Antitoxin

• AlphaFold DB: Q83AB4