Detailed information of TA system TA111958

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): spoIISABC/SpoIISA-SpoIISB
• Location: 3688659..3689578
• Replicon: chromosome
• Accession: NZ_CP032538
• Organism: Bacillus licheniformis strain MT-B06

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: T5HIF3
• Locus tag: D6022_RS19530
• Protein ID: WP_003180879.1
• Coordinates: 3688659..3689405 (+)
• Length: 249 a.a.

Antitoxin (Protein)

• Gene name: spoIISB
• Uniprot ID: Q65L00
• Locus tag: D6022_RS19535
• Protein ID: WP_011197812.1
• Coordinates: 3689405..3689578 (+)
• Length: 58 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D6022_RS19510	3684049..3684693	-	645	WP_009328721.1	YesL family protein	-
D6022_RS19515	3684830..3686587	-	1758	WP_017474396.1	gamma-glutamyltransferase	-
D6022_RS19520	3686920..3687537	+	618	WP_003180884.1	DUF47 domain-containing protein	-
D6022_RS19525	3687550..3688548	+	999	WP_009328723.1	inorganic phosphate transporter	-
D6022_RS19530	3688659..3689405	+	747	WP_003180879.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
D6022_RS19535	3689405..3689578	+	174	WP_011197812.1	type II toxin-antitoxin system SpoIISB family antitoxin	Antitoxin
D6022_RS19540	3689868..3691139	-	1272	WP_011197811.1	MFS transporter	-
D6022_RS19545	3691192..3692205	-	1014	WP_017474397.1	zinc-binding alcohol dehydrogenase family protein	-
D6022_RS19550	3692232..3693080	-	849	WP_009328728.1	SDR family oxidoreductase	-
D6022_RS19555	3693056..3694153	-	1098	WP_017474398.1	mannonate dehydratase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (3-238)

Antitoxin

• Antitoxin SpoIISB (1-57)

Sequences

Toxin

Protein

• Length: 249 a.a.
• Molecular weight: 29391.80 Da
• Isoelectric Point: 4.5367

>T111958 WP_003180879.1 NZ_CP032538:3688659-3689405 [Bacillus licheniformis]
MLLFYQFLVWLIVLALALYVAAVWRFEKQLAEKTVAIRKTWYLLYVIGAVIYWTHDPQSIFTNPLHYLIVAVFFTLTDAF
IFLNAYFKKLGSSELATDTRMLLEENNDLLHTYQNRLKTFQYLLKNEPIHIYYGNIEAYAEGIEKLIKRFAEKMNISAAL
CEYNSEESKDHLLEHMENRFDVQEKLDRKDVYYEENGKMVLIPFSIHDFDYVMKLTSEDLVTEFDYLLFTSLTSIYDLLL
PNEEEGDD

Nucleotide

• Length: 747 bp

>T111958 NZ_CP032538:3688659-3689405 [Bacillus licheniformis]
ATGCTGCTATTTTATCAATTCCTTGTTTGGCTTATTGTTTTGGCGCTGGCTCTGTACGTAGCTGCCGTGTGGCGTTTTGA
AAAACAGCTCGCCGAAAAAACGGTCGCCATCAGAAAAACGTGGTACCTTCTTTATGTCATCGGCGCCGTGATATATTGGA
CGCACGATCCGCAGTCAATTTTCACAAACCCGCTTCATTACCTGATCGTTGCCGTTTTTTTCACGTTGACAGACGCTTTT
ATTTTCTTAAATGCCTACTTTAAAAAGCTCGGCAGCTCTGAACTCGCGACAGATACAAGAATGCTTCTCGAAGAAAACAA
CGACCTCCTCCACACGTATCAAAACAGGCTGAAAACGTTTCAATACCTATTGAAAAACGAACCGATCCACATCTATTATG
GAAATATAGAAGCGTATGCAGAAGGCATCGAAAAGCTCATCAAACGGTTTGCCGAAAAAATGAATATATCCGCTGCACTT
TGCGAATATAATTCAGAAGAAAGCAAGGATCATTTGCTTGAGCATATGGAAAACCGGTTCGATGTTCAAGAAAAGCTTGA
CCGCAAAGACGTTTATTATGAAGAAAACGGAAAAATGGTCCTCATTCCTTTTTCCATCCACGATTTTGACTATGTCATGA
AGTTAACCTCAGAAGACCTTGTCACAGAATTTGATTATCTCCTGTTTACTTCTTTAACAAGCATCTATGATCTTCTGCTA
CCAAACGAAGAGGAAGGTGACGACTGA

Antitoxin

Protein

• Length: 58 a.a.
• Molecular weight: 6971.08 Da
• Isoelectric Point: 10.7815

>AT111958 WP_011197812.1 NZ_CP032538:3689405-3689578 [Bacillus licheniformis]
MEPAFQQDQESVKKRLAFKMFKRQSKTNIAKYEVSPYTERIFRQNERLIGEYKRKKA

Nucleotide

• Length: 174 bp

>AT111958 NZ_CP032538:3689405-3689578 [Bacillus licheniformis]
ATGGAGCCTGCTTTTCAGCAAGACCAGGAAAGCGTAAAAAAGCGCCTCGCCTTTAAAATGTTTAAAAGGCAATCCAAAAC
CAATATCGCGAAATACGAAGTCAGCCCCTATACCGAACGGATTTTCCGGCAAAACGAACGGCTGATCGGCGAGTATAAAA
GAAAAAAAGCTTAA

Structures

Toxin

• AlphaFold DB: T5HIF3

Antitoxin

• AlphaFold DB: Q65L00