Detailed information of TA system TA111956

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 1271995..1272631
• Replicon: chromosome
• Accession: NZ_CP032538
• Organism: Bacillus licheniformis strain MT-B06

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: M5P3Q9
• Locus tag: D6022_RS06420
• Protein ID: WP_003179128.1
• Coordinates: 1272281..1272631 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: M5PDU2
• Locus tag: D6022_RS06415
• Protein ID: WP_006638778.1
• Coordinates: 1271995..1272276 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D6022_RS06395	1267110..1268591	+	1482	WP_009330132.1	PH domain-containing protein	-
D6022_RS06400	1268588..1269187	-	600	WP_003179118.1	rhomboid family intramembrane serine protease	-
D6022_RS06405	1269530..1270489	+	960	WP_152847888.1	outer membrane lipoprotein carrier protein LolA	-
D6022_RS06410	1270714..1271883	+	1170	WP_026080763.1	alanine racemase	-
D6022_RS06415	1271995..1272276	+	282	WP_006638778.1	hypothetical protein	Antitoxin
D6022_RS06420	1272281..1272631	+	351	WP_003179128.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
D6022_RS06425	1272749..1273576	+	828	WP_003179130.1	RsbT co-antagonist protein RsbRA	-
D6022_RS06430	1273580..1273945	+	366	WP_003179132.1	STAS domain-containing protein	-
D6022_RS06435	1273948..1274349	+	402	WP_003179135.1	anti-sigma regulatory factor	-
D6022_RS06440	1274360..1275367	+	1008	WP_009330124.1	PP2C family protein-serine/threonine phosphatase	-
D6022_RS06445	1275426..1275752	+	327	WP_003179140.1	anti-sigma factor antagonist	-
D6022_RS06450	1275752..1276237	+	486	WP_003179142.1	anti-sigma B factor RsbW	-
D6022_RS06455	1276203..1276994	+	792	WP_003179144.1	RNA polymerase sigma factor SigB	-
D6022_RS06460	1276991..1277590	+	600	WP_003179145.1	SpoIIE family protein phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (1-113)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12990.03 Da
• Isoelectric Point: 5.7234

>T111956 WP_003179128.1 NZ_CP032538:1272281-1272631 [Bacillus licheniformis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNNIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMEKVNEALQISLALIDF

Nucleotide

• Length: 351 bp

>T111956 NZ_CP032538:1272281-1272631 [Bacillus licheniformis]
TTGATTGTTAAACGCGGCGATGTTTATTTTGCTGACCTATCTCCTGTTGTTGGCTCAGAACAAGGCGGGGTGCGCCCGGT
TTTAGTGATTCAAAACAACATCGGCAATCGCTTCAGCCCAACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CAAAATTACCTACCCACGTCGAAATTGATGCGAAACGCTACGGTTTTGAAAGAGACTCCGTTATATTGCTCGAACAAATT
CGGACGATTGACAAGCAAAGATTAACGGACAAAATCACCCATCTCGATGATGAAATGATGGAAAAGGTCAACGAAGCCTT
ACAAATCAGTTTGGCACTCATTGATTTTTAA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10649.03 Da
• Isoelectric Point: 4.6920

>AT111956 WP_006638778.1 NZ_CP032538:1271995-1272276 [Bacillus licheniformis]
MSESSATTEILIRLPEALVSELDGVVMQDNGSRNELIYQATKMYLRERKNRQIRESMRRGYMEMAKINLNISSEAQFAEY
EAENTVERLVSGG

Nucleotide

• Length: 282 bp

>AT111956 NZ_CP032538:1271995-1272276 [Bacillus licheniformis]
TTGTCTGAATCCAGCGCAACAACTGAAATATTGATTCGCTTGCCAGAAGCTTTAGTATCAGAACTGGATGGTGTCGTCAT
GCAAGATAACGGGAGCAGAAATGAACTGATTTACCAAGCCACAAAAATGTATTTACGCGAACGCAAAAATCGACAAATTC
GGGAATCGATGAGACGCGGTTATATGGAGATGGCCAAGATCAATTTGAACATCTCTTCTGAGGCTCAATTTGCAGAGTAT
GAGGCTGAAAACACAGTAGAGCGCTTAGTAAGCGGAGGATAA

Structures

Toxin

• AlphaFold DB: A0A6I7FHI4

Antitoxin

• AlphaFold DB: M5PDU2