Detailed information of TA system TA111955

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazF-pemI/MazF(toxin)
• Location: 4993840..4994476
• Replicon: chromosome
• Accession: NZ_CP032527
• Organism: Bacillus megaterium NCT-2

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: D5DWT4
• Locus tag: OEA_RS25630
• Protein ID: WP_013055004.1
• Coordinates: 4993840..4994190 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: D5DWT3
• Locus tag: OEA_RS25635
• Protein ID: WP_013055003.1
• Coordinates: 4994195..4994476 (-)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
OEA_RS25595	4989426..4990220	-	795	WP_013055011.1	RNA polymerase sigma factor SigB	-
OEA_RS25600	4990186..4990671	-	486	WP_016762758.1	anti-sigma B factor RsbW	-
OEA_RS25605	4990668..4991000	-	333	WP_013055009.1	anti-sigma factor antagonist	-
OEA_RS25610	4991060..4992070	-	1011	WP_013055008.1	PP2C family protein-serine/threonine phosphatase	-
OEA_RS25615	4992084..4992485	-	402	WP_013055007.1	anti-sigma regulatory factor	-
OEA_RS25620	4992489..4992845	-	357	WP_013055006.1	STAS domain-containing protein	-
OEA_RS25625	4992848..4993684	-	837	WP_013055005.1	STAS domain-containing protein	-
OEA_RS25630	4993840..4994190	-	351	WP_013055004.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
OEA_RS25635	4994195..4994476	-	282	WP_013055003.1	hypothetical protein	Antitoxin
OEA_RS25640	4994677..4995867	-	1191	WP_016762757.1	alanine racemase	-
OEA_RS25645	4995981..4997039	-	1059	WP_016762756.1	outer membrane lipoprotein carrier protein LolA	-
OEA_RS25650	4997098..4997463	-	366	WP_026106481.1	holo-ACP synthase	-
OEA_RS25655	4997528..4998130	+	603	WP_013081417.1	rhomboid family intramembrane serine protease	-
OEA_RS25660	4998139..4999110	-	972	WP_016762754.1	UV DNA damage repair endonuclease UvsE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12991.96 Da
• Isoelectric Point: 4.8781

>T111955 WP_013055004.1 NZ_CP032527:c4994190-4993840 [Bacillus megaterium NCT-2]
MVVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDRVDEALQISVGLIDF

Nucleotide

• Length: 351 bp

>T111955 NZ_CP032527:c4994190-4993840 [Bacillus megaterium NCT-2]
TTGGTAGTCAAACGTGGCGACGTTTATTTTGCTGACCTATCTCCTGTTGTTGGCTCAGAACAAGGAGGCGTTCGCCCTGT
ACTTGTCATTCAAAATGATATAGGCAATCGTTTTAGCCCAACTGTTATTGTAGCTGCGATCACTGCTCAAATTCAAAAGG
CAAAGTTACCTACGCATGTTGAAATTGATGCAAAGCGCTATGGATTTGAGCGAGATTCAGTTATTTTACTAGAACAAATT
CGTACTATTGATAAACAGAGGCTAACAGACAAGATTACCCATTTAGATGATGAAATGATGGACAGAGTGGATGAAGCCTT
GCAAATCAGTGTAGGACTTATCGACTTTTAG

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10691.22 Da
• Isoelectric Point: 8.5339

>AT111955 WP_013055003.1 NZ_CP032527:c4994476-4994195 [Bacillus megaterium NCT-2]
VSEASATTEILVRLPKNLVSELDLMVKQENGNRSDFIHQATKLYLRERKKRHIRESMRRGYMEMAKINLNIASEAFLAEY
EADHTVDRLVSGG

Nucleotide

• Length: 282 bp

>AT111955 NZ_CP032527:c4994476-4994195 [Bacillus megaterium NCT-2]
GTGTCTGAAGCGAGCGCAACTACAGAAATCCTAGTAAGATTACCGAAAAATTTAGTATCGGAGCTCGATCTTATGGTTAA
ACAAGAAAATGGCAACCGAAGTGATTTTATTCACCAAGCAACAAAATTGTACCTTCGTGAGCGAAAAAAACGCCATATTC
GCGAATCAATGAGACGTGGATATATGGAGATGGCAAAGATAAACTTAAATATTGCTTCCGAGGCTTTTCTAGCAGAGTAT
GAAGCTGATCATACTGTGGATCGTTTAGTAAGCGGGGGGTAA

Structures

Antitoxin

• AlphaFold DB: A0A0B6AMB9