Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 2677600..2678218 Replicon chromosome
Accession NZ_CP032523
Organism Shigella sonnei strain AR_0030

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag AM357_RS13650 Protein ID WP_001291435.1
Coordinates 2677600..2677818 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag AM357_RS13655 Protein ID WP_000344800.1
Coordinates 2677844..2678218 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AM357_RS13615 (2672892) 2672892..2673464 + 573 WP_000779812.1 YbaY family lipoprotein -
AM357_RS13620 (2673495) 2673495..2673806 - 312 WP_167553807.1 MGMT family protein -
AM357_RS13630 (2674185) 2674185..2674538 + 354 WP_000878140.1 DUF1428 domain-containing protein -
AM357_RS13635 (2674580) 2674580..2676128 - 1549 Protein_2633 cyclic-guanylate-specific phosphodiesterase PdeB -
AM357_RS13640 (2676292) 2676292..2676762 - 471 WP_000136192.1 YlaC family protein -
AM357_RS13645 (2676878) 2676878..2677429 - 552 WP_000102564.1 maltose O-acetyltransferase -
AM357_RS13650 (2677600) 2677600..2677818 - 219 WP_001291435.1 HHA domain-containing protein Toxin
AM357_RS13655 (2677844) 2677844..2678218 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
AM357_RS13660 (2678764) 2678764..2681913 - 3150 WP_001132464.1 efflux RND transporter permease AcrB -
AM357_RS13665 (2681936) 2681936..2683129 - 1194 WP_011310152.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T111940 WP_001291435.1 NZ_CP032523:c2677818-2677600 [Shigella sonnei]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T111940 NZ_CP032523:c2677818-2677600 [Shigella sonnei]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTATACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT111940 WP_000344800.1 NZ_CP032523:c2678218-2677844 [Shigella sonnei]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT111940 NZ_CP032523:c2678218-2677844 [Shigella sonnei]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References