Detailed information of TA system TA111939

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 2642039..2642876
• Replicon: chromosome
• Accession: NZ_CP032523
• Organism: Shigella sonnei strain AR_0030

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: Q3Z4X7
• Locus tag: AM357_RS13475
• Protein ID: WP_000227784.1
• Coordinates: 2642039..2642581 (-)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: I2UQS9
• Locus tag: AM357_RS13480
• Protein ID: WP_001297137.1
• Coordinates: 2642565..2642876 (-)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM357_RS13445 (2637103)	2637103..2637594	+	492	WP_001138904.1	nucleotide binding protein YajQ	-
AM357_RS13450 (2637722)	2637722..2639086	-	1365	WP_001000971.1	MFS transporter	-
AM357_RS13455 (2639205)	2639205..2639902	+	698	WP_237158176.1	IS1-like element IS1A family transposase	-
AM357_RS13460 (2640271)	2640271..2640669	+	399	Protein_2598	SEL1-like repeat protein	-
AM357_RS13470 (2641417)	2641417..2641983	+	567	Protein_2600	SEL1-like repeat protein	-
AM357_RS13475 (2642039)	2642039..2642581	-	543	WP_000227784.1	GNAT family N-acetyltransferase	Toxin
AM357_RS13480 (2642565)	2642565..2642876	-	312	WP_001297137.1	DUF1778 domain-containing protein	Antitoxin
AM357_RS13485 (2643061)	2643061..2643951	-	891	WP_000971327.1	heme o synthase	-
AM357_RS13490 (2643963)	2643963..2644292	-	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
AM357_RS13495 (2644292)	2644292..2644906	-	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
AM357_RS13500 (2644896)	2644896..2646887	-	1992	WP_000467180.1	cytochrome o ubiquinol oxidase subunit I	-
AM357_RS13505 (2646909)	2646909..2647856	-	948	WP_001239441.1	cytochrome o ubiquinol oxidase subunit II	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (68-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-97)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19805.02 Da
• Isoelectric Point: 8.3395

>T111939 WP_000227784.1 NZ_CP032523:c2642581-2642039 [Shigella sonnei]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T111939 NZ_CP032523:c2642581-2642039 [Shigella sonnei]
ATGGTCGACAAGCATGAAGAGATTACTCTGCCCATAGTCCTCTCCTGTAATTATCAGTCTGATATTACTTATCCTGGGCA
AAAACAGTTTGATTGCGGTAACCCTGTTATCGATAAATTTGTACGCGCATCGCTAAAGAAAAGTGTGCGTAATAGCGACT
GTGCGGCTAAAGCACTTATTGACAGACAAAGTGGTGAACTGATCGGCATCTGTACTTTTACGGCATATTCGCTGGAAAAA
CAACGCGTTTCTGGCGTCCTTCAGGGTTCACAACCTTCAGAAATTGGTGTTGTCAGATTAGTCATGTTGGGGGTAGCACG
GAAGTATCAAAAGCGGGGCTTTGGTCAGGACCTACTATGTGATTTTTTTGAACATGTAAAAATAATTCACCAGGCATTAC
CAATTAAAGGGGTTTATCTTGATGCTGACCCTGCCGCCATTAATTTTTATGCTCGTCTCGGCTTTGTTCAGCTTTCAGCG
ACACCAAATGCTTTTGGTGCTGTACCTATGTTTTTGGCGATTCAGCATATTCTCGCGGCTTAG

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11358.04 Da
• Isoelectric Point: 10.4825

>AT111939 WP_001297137.1 NZ_CP032523:c2642876-2642565 [Shigella sonnei]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT111939 NZ_CP032523:c2642876-2642565 [Shigella sonnei]
ATGCCTGGAAAAACAGCCACTCTACCCGATGTCGATAAAACGCTGAAAAATGCGCGGGTCGAACTCAAAACTAGTCCCGA
CGCCAAAAACAAACTTCGTGAAGCTGCGCAAGCCGTTGGGGTAGATTTAAGCGCTTTTATTTTGAGCGCTGCTATGGAAC
GCGCCGAAAGTGTGCTTGATAACCAACGTCGCCGTGAGCTTTCGAATCAAAGCTGGGAACTGATGAACCAACTCATCGCT
GAACCTGCTCAACCGACGCTCGCCCTCAAGGCGTTAATGAAAAGGAAAAACAGCGATGGTCGACAAGCATGA

Structures

Toxin

• PDB: 7BYY

Antitoxin

• AlphaFold DB: A0A0E0Y6B3