Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 1903985..1904482 Replicon chromosome
Accession NZ_CP032523
Organism Shigella sonnei strain AR_0030

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag AM357_RS09790 Protein ID WP_001173343.1
Coordinates 1904309..1904482 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag AM357_RS09785 Protein ID WP_000398619.1
Coordinates 1903985..1904281 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AM357_RS09765 (1900716) 1900716..1901435 - 720 WP_000611285.1 two-component system response regulator DcuR -
AM357_RS09770 (1901432) 1901432..1903063 - 1632 WP_001216495.1 sensor histidine kinase -
AM357_RS09775 (1903244) 1903244..1903474 + 231 WP_000371698.1 4Fe-4S mono-cluster protein YjdI -
AM357_RS09780 (1903486) 1903486..1903758 + 273 WP_000405647.1 GNAT family N-acetyltransferase -
AM357_RS09785 (1903985) 1903985..1904281 + 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
AM357_RS09790 (1904309) 1904309..1904482 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
AM357_RS09795 (1904601) 1904601..1906118 - 1518 WP_001295086.1 lysine--tRNA ligase -
AM357_RS09800 (1906355) 1906355..1907812 - 1458 WP_000856831.1 dipeptide/tripeptide permease DtpC -
AM357_RS09805 (1907871) 1907871..1908128 - 258 Protein_1877 lysine decarboxylase LdcC -
AM357_RS09815 (1908174) 1908174..1908884 - 711 Protein_1878 IS3-like element IS600 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T111937 WP_001173343.1 NZ_CP032523:1904309-1904482 [Shigella sonnei]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T111937 NZ_CP032523:1904309-1904482 [Shigella sonnei]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT111937 WP_000398619.1 NZ_CP032523:1903985-1904281 [Shigella sonnei]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT111937 NZ_CP032523:1903985-1904281 [Shigella sonnei]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCTTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References