Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 1154246..1154874 Replicon chromosome
Accession NZ_CP032523
Organism Shigella sonnei strain AR_0030

Toxin (Protein)


Gene name hicA Uniprot ID A0A827WKV6
Locus tag AM357_RS06140 Protein ID WP_001259380.1
Coordinates 1154602..1154874 (-) Length 91 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag AM357_RS06135 Protein ID WP_000593555.1
Coordinates 1154246..1154605 (-) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AM357_RS06110 (1150378) 1150378..1151322 + 945 WP_000947068.1 nickel ABC transporter permease subunit NikB -
AM357_RS06115 (1151319) 1151319..1152152 + 834 WP_001008963.1 nickel ABC transporter permease subunit NikC -
AM357_RS06120 (1152152) 1152152..1152916 + 765 WP_001136223.1 nickel import ATP-binding protein NikD -
AM357_RS06125 (1152913) 1152913..1153719 + 807 WP_000173703.1 nickel import ATP-binding protein NikE -
AM357_RS06130 (1153725) 1153725..1154126 + 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
AM357_RS06135 (1154246) 1154246..1154605 - 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
AM357_RS06140 (1154602) 1154602..1154874 - 273 WP_001259380.1 type II toxin-antitoxin system HicA family toxin Toxin
AM357_RS06145 (1154947) 1154947..1156071 - 1125 WP_005140446.1 ABC transporter permease -
AM357_RS06150 (1156071) 1156071..1158806 - 2736 WP_000149089.1 ribosome-associated ATPase/putative transporter RbbA -
AM357_RS06155 (1158803) 1158803..1159870 - 1068 WP_000361520.1 HlyD family secretion protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(29-79)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 91 a.a.        Molecular weight: 10071.81 Da        Isoelectric Point: 10.7076

>T111936 WP_001259380.1 NZ_CP032523:c1154874-1154602 [Shigella sonnei]
MRTKVQALRKKKNTLDQIFKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESVR
DWLLSIGVKP

Download         Length: 273 bp

>T111936 NZ_CP032523:c1154874-1154602 [Shigella sonnei]
ATGAGAACCAAGGTACAGGCTTTACGGAAGAAAAAAAATACATTGGATCAAATATTTAAAACTCCTGTTCCTCAAGGGAT
CAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGTAAGTTCA
TACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTGCGT
GACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT111936 WP_000593555.1 NZ_CP032523:c1154605-1154246 [Shigella sonnei]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT111936 NZ_CP032523:c1154605-1154246 [Shigella sonnei]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCAGCTGTTATTACCTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A827WKV6

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References