Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 550265..550919 Replicon chromosome
Accession NZ_CP032523
Organism Shigella sonnei strain AR_0030

Toxin (Protein)


Gene name cptA Uniprot ID -
Locus tag AM357_RS02990 Protein ID WP_000244766.1
Coordinates 550265..550672 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag AM357_RS02995 Protein ID WP_000354046.1
Coordinates 550653..550919 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AM357_RS02970 (546222) 546222..547955 - 1734 WP_000813167.1 single-stranded-DNA-specific exonuclease RecJ -
AM357_RS02975 (547961) 547961..548671 - 711 WP_000715206.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
AM357_RS02980 (548696) 548696..549592 - 897 WP_134812137.1 site-specific tyrosine recombinase XerD -
AM357_RS02985 (549704) 549704..550225 + 522 WP_001055874.1 flavodoxin FldB -
AM357_RS02990 (550265) 550265..550672 - 408 WP_000244766.1 protein YgfX Toxin
AM357_RS02995 (550653) 550653..550919 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
AM357_RS03000 (551162) 551162..552142 + 981 WP_000886060.1 tRNA-modifying protein YgfZ -
AM357_RS03005 (552338) 552338..552997 - 660 WP_000250274.1 hemolysin III family protein -
AM357_RS03010 (553161) 553161..553472 - 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
AM357_RS03015 (553517) 553517..554950 + 1434 WP_005137189.1 6-phospho-beta-glucosidase BglA -
AM357_RS03020 (555007) 555007..555750 - 744 WP_052989136.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 15932.82 Da        Isoelectric Point: 11.2669

>T111933 WP_000244766.1 NZ_CP032523:c550672-550265 [Shigella sonnei]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVGSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T111933 NZ_CP032523:c550672-550265 [Shigella sonnei]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTGGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT111933 WP_000354046.1 NZ_CP032523:c550919-550653 [Shigella sonnei]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT111933 NZ_CP032523:c550919-550653 [Shigella sonnei]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References