Detailed information of TA system TA111927

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazE-MazF
• Location: 2058460..2059022
• Replicon: chromosome
• Accession: NZ_CP032519
• Organism: Cupriavidus oxalaticus strain T2

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: D2917_RS22170
• Protein ID: WP_151071977.1
• Coordinates: 2058460..2058708 (+)
• Length: 83 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: A0A375FL37
• Locus tag: D2917_RS22175
• Protein ID: WP_063237929.1
• Coordinates: 2058702..2059022 (+)
• Length: 107 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D2917_RS22140	2053857..2054432	-	576	WP_151071972.1	ribosomal protein S18-alanine N-acetyltransferase	-
D2917_RS22145	2054429..2055187	-	759	WP_167525704.1	tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex dimerization subunit type 1 TsaB	-
D2917_RS22150	2055307..2055576	+	270	WP_151071973.1	acyl-CoA-binding protein	-
D2917_RS22155	2055605..2057086	-	1482	WP_151071974.1	DHA2 family efflux MFS transporter permease subunit	-
D2917_RS22160	2057083..2057553	-	471	WP_151071975.1	DUF1857 family protein	-
D2917_RS22165	2057900..2058307	+	408	WP_151071976.1	VOC family protein	-
D2917_RS22170	2058460..2058708	+	249	WP_151071977.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Toxin
D2917_RS22175	2058702..2059022	+	321	WP_063237929.1	type II toxin-antitoxin system PemK/MazF family toxin	Antitoxin
D2917_RS22180	2059222..2060034	+	813	WP_151071978.1	MetQ/NlpA family ABC transporter substrate-binding protein	-
D2917_RS22195	2060472..2060690	-	219	WP_151071979.1	DUF1484 family protein	-
D2917_RS22200	2061030..2062571	+	1542	WP_151071980.1	serine hydrolase	-
D2917_RS22205	2062614..2063489	+	876	WP_151071982.1	metal-dependent hydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• mRNA interferase PemK-like (1-101)

Sequences

Toxin

Protein

• Length: 83 a.a.
• Molecular weight: 9074.36 Da
• Isoelectric Point: 4.3322

>T111927 WP_151071977.1 NZ_CP032519:2058460-2058708 [Cupriavidus oxalaticus]
MKATIRKMGNSQGILIPKTILAQLGLEDEVEMDIVNDTLVIRRPRNTPREGWAEASRAIAEAGDDVLVLGEFPNADDAEL
KW

Nucleotide

• Length: 249 bp

>T111927 NZ_CP032519:2058460-2058708 [Cupriavidus oxalaticus]
ATGAAAGCAACGATCCGAAAAATGGGCAACTCGCAGGGGATCTTGATCCCCAAGACAATCCTGGCGCAACTGGGTCTGGA
GGATGAAGTGGAAATGGATATCGTGAACGACACGCTGGTGATTCGGCGCCCGAGGAATACGCCGCGCGAAGGCTGGGCCG
AAGCCAGCCGCGCCATTGCCGAAGCAGGCGACGATGTGCTGGTCCTGGGCGAGTTTCCCAACGCGGACGATGCGGAGCTG
AAATGGTAG

Antitoxin

Protein

• Length: 107 a.a.
• Molecular weight: 11909.93 Da
• Isoelectric Point: 10.4436

>AT111927 WP_063237929.1 NZ_CP032519:2058702-2059022 [Cupriavidus oxalaticus]
MVVRGEVWLVALDPAAGSEIRKTRPCVVVSPPEMHDYLRTVTVAPMTSGSRPAPFRVGVTFQRKHGLILLDQIRAVDKSR
LLKRAGELNENTLRETLRVLRECFAD

Nucleotide

• Length: 321 bp

>AT111927 NZ_CP032519:2058702-2059022 [Cupriavidus oxalaticus]
ATGGTAGTGCGGGGTGAGGTCTGGCTTGTCGCCCTTGACCCAGCTGCGGGCAGTGAAATCAGGAAGACGCGCCCGTGCGT
CGTCGTCTCGCCACCGGAGATGCATGACTACCTCCGAACAGTCACCGTTGCCCCCATGACGTCCGGTAGCCGGCCCGCAC
CCTTTCGGGTCGGCGTGACGTTCCAGCGTAAACACGGGCTGATTCTCCTCGACCAGATTCGTGCCGTCGATAAATCCCGA
CTACTGAAGCGCGCTGGAGAGTTGAATGAGAACACGCTACGGGAGACCCTCCGCGTCTTGCGCGAGTGCTTCGCGGATTA
A

Structures

Antitoxin

• AlphaFold DB: A0A375FL37