111920

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-RelB
Location 32817..33424 Replicon plasmid pEco118UIb
Accession NZ_CP032516
Organism Escherichia coli strain 118UI

Toxin (Protein)


Gene name doc Uniprot ID B7LIH8
Locus tag Eco118UI_RS26185 Protein ID WP_000673985.1
Coordinates 33038..33424 (+) Length 129 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID B7LIH9
Locus tag Eco118UI_RS26180 Protein ID WP_000493532.1
Coordinates 32817..33041 (+) Length 75 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
Eco118UI_RS26160 29052..29399 + 348 WP_023144239.1 metalloregulator ArsR/SmtB family transcription factor -
Eco118UI_RS26165 29941..30150 + 210 WP_024192676.1 hypothetical protein -
Eco118UI_RS26895 30216..30470 - 255 Protein_28 hypothetical protein -
Eco118UI_RS26170 30563..31735 + 1173 Protein_29 IS21 family transposase -
Eco118UI_RS26175 31735..32532 + 798 WP_021557728.1 IS21-like element IS21 family helper ATPase IstB -
Eco118UI_RS26180 32817..33041 + 225 WP_000493532.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
Eco118UI_RS26185 33038..33424 + 387 WP_000673985.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
Eco118UI_RS26190 34202..35926 - 1725 WP_109866728.1 ABC transporter ATP-binding protein/permease -
Eco118UI_RS26195 35926..36608 - 683 Protein_34 lasso peptide biosynthesis B2 protein -
Eco118UI_RS26200 36608..37997 - 1390 Protein_35 hypothetical protein -
Eco118UI_RS26205 38125..38313 - 189 WP_000744815.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid tet(A) / mph(A) / sul1 / qacE / aadA5 senB 1..90328 90328


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Fido domain

(11-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-52)


Sequences

Toxin        


Download         Length: 129 a.a.        Molecular weight: 14370.62 Da        Isoelectric Point: 7.0732

>T111920 WP_000673985.1 NZ_CP032516:33038-33424 [Escherichia coli]
MKFVSSQEVIEFHDRLISRDGGVAGMPEPGRADAIIHRVLNMYHYEGVTDIMDLAAVYLVAIARGHIFNDANKRTALFVA
QVFLKRNGVHIISSRISFDEMQIIALNAATGEYNWKMVSDHLKAIILN

Download         Length: 387 bp

>T111920 NZ_CP032516:33038-33424 [Escherichia coli]
ATGAAATTCGTTAGTTCACAGGAAGTGATTGAGTTTCATGACCGCTTGATTTCGCGTGATGGTGGCGTGGCGGGTATGCC
AGAACCCGGTCGCGCTGACGCGATCATTCATCGTGTTTTGAACATGTATCATTATGAAGGTGTGACAGATATTATGGATC
TGGCTGCCGTGTATTTAGTTGCTATTGCTCGTGGTCACATTTTCAACGATGCAAATAAGCGAACAGCCTTGTTTGTTGCA
CAGGTTTTTTTAAAACGGAATGGTGTACATATTATTAGTTCACGTATTTCGTTTGATGAAATGCAAATTATCGCTTTGAA
TGCTGCAACAGGAGAATATAACTGGAAGATGGTTTCTGACCATCTTAAAGCCATTATTCTAAATTAG

Antitoxin


Download         Length: 75 a.a.        Molecular weight: 8361.39 Da        Isoelectric Point: 5.6713

>AT111920 WP_000493532.1 NZ_CP032516:32817-33041 [Escherichia coli]
MFTCNYSHARDNLASLLELAVDENQPVEIQRRKGGAAVLVDKDAFERYRKAALDAEFAEIMQSHGTAYTELSKR

Download         Length: 225 bp

>AT111920 NZ_CP032516:32817-33041 [Escherichia coli]
ATGTTTACATGTAACTATAGCCATGCTCGCGATAACCTGGCCTCGTTGCTCGAGCTGGCGGTTGATGAAAATCAGCCTGT
TGAGATTCAGCGCCGTAAAGGTGGCGCGGCTGTACTAGTTGATAAAGACGCTTTTGAACGATACCGTAAAGCGGCTCTTG
ATGCTGAGTTTGCTGAGATCATGCAAAGTCATGGGACGGCCTATACGGAGCTTTCTAAGCGATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A086V9Y9

Antitoxin

Source ID Structure
AlphaFold DB A0A086V9Y8

References