Detailed information of TA system TA111909

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 3546693..3547527
• Replicon: chromosome
• Accession: NZ_CP032515
• Organism: Escherichia coli strain 118UI

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A8E0IX31
• Locus tag: Eco118UI_RS17810
• Protein ID: WP_000854689.1
• Coordinates: 3547150..3547527 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A8E0IVP8
• Locus tag: Eco118UI_RS17805
• Protein ID: WP_001285599.1
• Coordinates: 3546693..3547073 (+)
• Length: 127 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
Eco118UI_RS17770	3542780..3543235	+	456	WP_001309747.1	hypothetical protein	-
Eco118UI_RS26930	3543336..3543467	+	132	WP_106108514.1	DUF905 family protein	-
Eco118UI_RS17780	3543646..3544467	+	822	WP_001234555.1	DUF945 domain-containing protein	-
Eco118UI_RS17785	3544731..3545204	+	474	WP_001538139.1	antirestriction protein	-
Eco118UI_RS17790	3545220..3545696	+	477	WP_001186761.1	RadC family protein	-
Eco118UI_RS17795	3545759..3545980	+	222	WP_000692315.1	DUF987 domain-containing protein	-
Eco118UI_RS17800	3545999..3546643	+	645	WP_000086757.1	hypothetical protein	-
Eco118UI_RS17805	3546693..3547073	+	381	WP_001285599.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
Eco118UI_RS17810	3547150..3547527	+	378	WP_000854689.1	TA system toxin CbtA family protein	Toxin
Eco118UI_RS17815	3547524..3548012	+	489	WP_000761698.1	hypothetical protein	-
Eco118UI_RS17820	3548029..3548226	+	198	WP_000839250.1	DUF957 domain-containing protein	-
Eco118UI_RS17825	3548311..3549156	+	846	WP_001446750.1	DUF4942 domain-containing protein	-
Eco118UI_RS17830	3549225..3549620	+	396	WP_000208381.1	type IV toxin-antitoxin system AbiEi family antitoxin domain-containing protein	-
Eco118UI_RS17835	3549613..3550546	+	934	Protein_3379	nucleotidyl transferase AbiEii/AbiGii toxin family protein	-
Eco118UI_RS26860	3550978..3551133	+	156	WP_000729638.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	3550978..3551133	155

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14042.07 Da
• Isoelectric Point: 9.1510

>T111909 WP_000854689.1 NZ_CP032515:3547150-3547527 [Escherichia coli]
MKTLPDTHVRAASRCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SAGAPSQLINSIDILRARRATGLMTRNNYRMVNNITQGKHPEAKR

Nucleotide

• Length: 378 bp

>T111909 NZ_CP032515:3547150-3547527 [Escherichia coli]
ATGAAAACATTACCCGACACACACGTACGGGCGGCATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTATGGCCTCACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGGATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGTACCGACCAGCCGGGATTC
AGCGCAGGAGCCCCGTCGCAGTTAATCAACAGCATTGATATTCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGGAA
CAATTACAGAATGGTAAATAACATTACCCAGGGCAAGCATCCGGAGGCAAAACGATGA

Antitoxin

Protein

• Length: 127 a.a.
• Molecular weight: 13984.78 Da
• Isoelectric Point: 5.0823

>AT111909 WP_001285599.1 NZ_CP032515:3546693-3547073 [Escherichia coli]
MSDTLPGTTLPDDNKDRPWWGLPCTVTPCFGARLLQEGNRLHYLADRAGIRGRFSNADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCDADTLGSCGYVYLAVYPATETESNPPE

Nucleotide

• Length: 381 bp

>AT111909 NZ_CP032515:3546693-3547073 [Escherichia coli]
ATGTCGGACACACTCCCCGGAACAACGCTTCCCGACGACAACAAAGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGCGCCCGTCTGTTGCAGGAGGGTAACCGGTTGCATTACCTTGCAGACCGCGCCGGTATCAGGGGCCGGT
TCAGCAATGCGGATGCATACCATCTGGACCAGGCTTTTCCACTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCGAAAGGGCTGACCTGCGATGCCGACACCCTCGGGTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCTGCGACCGAAACGGAAAGTAACCCTCCGGAATAG