Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3383758..3384412 Replicon chromosome
Accession NZ_CP032515
Organism Escherichia coli strain 118UI

Toxin (Protein)


Gene name cptA Uniprot ID B7N7E0
Locus tag Eco118UI_RS16895 Protein ID WP_000244769.1
Coordinates 3383758..3384165 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag Eco118UI_RS16900 Protein ID WP_000354046.1
Coordinates 3384146..3384412 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
Eco118UI_RS16875 3379715..3381448 - 1734 WP_000813190.1 single-stranded-DNA-specific exonuclease RecJ -
Eco118UI_RS16880 3381454..3382164 - 711 WP_000715210.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
Eco118UI_RS16885 3382189..3383085 - 897 WP_000806652.1 site-specific tyrosine recombinase XerD -
Eco118UI_RS16890 3383197..3383718 + 522 WP_001055874.1 flavodoxin FldB -
Eco118UI_RS16895 3383758..3384165 - 408 WP_000244769.1 protein YgfX Toxin
Eco118UI_RS16900 3384146..3384412 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
Eco118UI_RS16905 3384655..3385635 + 981 WP_000886049.1 tRNA-modifying protein YgfZ -
Eco118UI_RS16910 3385712..3386371 - 660 WP_000250274.1 hemolysin III family protein -
Eco118UI_RS16915 3386535..3386846 - 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
Eco118UI_RS16920 3386891..3388324 + 1434 WP_001513393.1 6-phospho-beta-glucosidase BglA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16062.03 Da        Isoelectric Point: 11.5554

>T111908 WP_000244769.1 NZ_CP032515:c3384165-3383758 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGK
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T111908 NZ_CP032515:c3384165-3383758 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCGCAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGCATTAATGCTCGCCAGGGGGAAATTCGCCTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGAAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGTGGCATGATGCTGCGTTTACGTTCTGATAGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT111908 WP_000354046.1 NZ_CP032515:c3384412-3384146 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT111908 NZ_CP032515:c3384412-3384146 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAGTGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCAGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829J3T1

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References