Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3037773..3038427 Replicon chromosome
Accession NZ_CP032513
Organism Shigella flexneri strain SFL1520

Toxin (Protein)


Gene name cptA Uniprot ID Q0T101
Locus tag D6T62_RS16090 Protein ID WP_000244767.1
Coordinates 3038020..3038427 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID Q0T100
Locus tag D6T62_RS16085 Protein ID WP_000354044.1
Coordinates 3037773..3038039 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
D6T62_RS16060 (3032943) 3032943..3033686 + 744 WP_000951959.1 SDR family oxidoreductase -
D6T62_RS16065 (3033743) 3033743..3035175 - 1433 Protein_3078 6-phospho-beta-glucosidase BglA -
D6T62_RS16070 (3035220) 3035220..3035531 + 312 WP_001182950.1 N(4)-acetylcytidine aminohydrolase -
D6T62_RS16075 (3035695) 3035695..3036354 + 660 WP_000250274.1 hemolysin III family protein -
D6T62_RS16080 (3036550) 3036550..3037530 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
D6T62_RS16085 (3037773) 3037773..3038039 + 267 WP_000354044.1 FAD assembly factor SdhE Antitoxin
D6T62_RS16090 (3038020) 3038020..3038427 + 408 WP_000244767.1 protein YgfX Toxin
D6T62_RS16095 (3038467) 3038467..3038988 - 522 WP_001055867.1 flavodoxin FldB -
D6T62_RS16100 (3039100) 3039100..3039996 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
D6T62_RS16105 (3040021) 3040021..3040731 + 711 WP_000715213.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
D6T62_RS16110 (3040737) 3040737..3042470 + 1734 WP_000813187.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 15978.91 Da        Isoelectric Point: 10.9373

>T111883 WP_000244767.1 NZ_CP032513:3038020-3038427 [Shigella flexneri]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRCINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T111883 NZ_CP032513:3038020-3038427 [Shigella flexneri]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTATTTGATTGCG
TTCGCAGCCAGCGGTGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10529.09 Da        Isoelectric Point: 5.1599

>AT111883 WP_000354044.1 NZ_CP032513:3037773-3038039 [Shigella flexneri]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEIMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT111883 NZ_CP032513:3037773-3038039 [Shigella flexneri]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATAATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TIU0

Antitoxin

Source ID Structure
AlphaFold DB A0A4P7TIU2

References