Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 56944..57581 Replicon chromosome
Accession NZ_CP032506
Organism Bacillus velezensis strain JT3-1

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag D5H27_RS00320 Protein ID WP_003156187.1
Coordinates 57231..57581 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID I2HMS5
Locus tag D5H27_RS00315 Protein ID WP_003156188.1
Coordinates 56944..57225 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
D5H27_RS00295 53309..53908 - 600 WP_032872997.1 rhomboid family intramembrane serine protease -
D5H27_RS00300 54001..54366 + 366 WP_007609580.1 holo-ACP synthase -
D5H27_RS00305 54531..55538 + 1008 WP_032872999.1 outer membrane lipoprotein carrier protein LolA -
D5H27_RS00310 55655..56824 + 1170 WP_032873001.1 alanine racemase -
D5H27_RS00315 56944..57225 + 282 WP_003156188.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
D5H27_RS00320 57231..57581 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
D5H27_RS00325 57699..58520 + 822 WP_003156182.1 STAS domain-containing protein -
D5H27_RS00330 58525..58890 + 366 WP_003156180.1 RsbT antagonist protein RsbS -
D5H27_RS00335 58893..59294 + 402 WP_003156178.1 anti-sigma regulatory factor -
D5H27_RS00340 59306..60313 + 1008 WP_007609589.1 PP2C family protein-serine/threonine phosphatase -
D5H27_RS00345 60377..60706 + 330 WP_003156176.1 anti-sigma factor antagonist RsbV -
D5H27_RS00350 60703..61185 + 483 WP_007609591.1 anti-sigma B factor RsbW -
D5H27_RS00355 61151..61939 + 789 WP_032873003.1 RNA polymerase sigma factor SigB -
D5H27_RS00360 61939..62541 + 603 WP_007410234.1 SpoIIE family protein phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T111860 WP_003156187.1 NZ_CP032506:57231-57581 [Bacillus velezensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

>T111860 NZ_CP032506:57231-57581 [Bacillus velezensis]
TTGATTGTAAAGCGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGCGTACGCCCGGT
TTTAGTGATCCAAAATGATATCGGAAATCGCTTTAGCCCGACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATTGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGATTAACGGATAAAATAACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCTT
GCAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10562.92 Da        Isoelectric Point: 6.4706

>AT111860 WP_003156188.1 NZ_CP032506:56944-57225 [Bacillus velezensis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Download         Length: 282 bp

>AT111860 NZ_CP032506:56944-57225 [Bacillus velezensis]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCTGAAAACTTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGGACAACTCGCCATAACC
GTGATTTGATGAGACGCGGGTATATGGAAATGGCGAAAATCAACTTGAATATCTCTTCTGAGGCTCACTATGCTGAGTGC
GAAGCAGAAACGACCGTTGAGCGCTTAGTCAGCGGAGGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
AlphaFold DB I2HMS5

References