Detailed information of TA system TA111853

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Hha-TomB/-
• Location: 2872891..2873509
• Replicon: chromosome
• Accession: NZ_CP032487
• Organism: Yersinia hibernica strain CFS1934

Toxin (Protein)

• Gene name: Hha
• Uniprot ID: Q66DR5
• Locus tag: D5F51_RS13625
• Protein ID: WP_002208622.1
• Coordinates: 2873306..2873509 (+)
• Length: 68 a.a.

Antitoxin (Protein)

• Gene name: TomB
• Uniprot ID: -
• Locus tag: D5F51_RS13620
• Protein ID: WP_025377437.1
• Coordinates: 2872891..2873259 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D5F51_RS13590	2868571..2868831	+	261	WP_025377443.1	type B 50S ribosomal protein L31	-
D5F51_RS13595	2868847..2868990	+	144	WP_002208618.1	type B 50S ribosomal protein L36	-
D5F51_RS13600	2869108..2869986	-	879	WP_087769094.1	metal ABC transporter substrate-binding protein	-
D5F51_RS13605	2870015..2870872	-	858	WP_129197319.1	metal ABC transporter permease	-
D5F51_RS13610	2870869..2871606	-	738	WP_129197321.1	metal ABC transporter ATP-binding protein	-
D5F51_RS13615	2872379..2872732	+	354	WP_129197324.1	hypothetical protein	-
D5F51_RS13620	2872891..2873259	+	369	WP_025377437.1	Hha toxicity modulator TomB	Antitoxin
D5F51_RS13625	2873306..2873509	+	204	WP_002208622.1	expression modulating protein YmoA	Toxin
D5F51_RS13630	2874424..2877615	-	3192	WP_206197183.1	inverse autotransporter beta domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (8-64)

Antitoxin

• Biofilm formation regulator YbaJ (1-118)

Sequences

Toxin

Protein

• Length: 68 a.a.
• Molecular weight: 8063.31 Da
• Isoelectric Point: 6.4573

>T111853 WP_002208622.1 NZ_CP032487:2873306-2873509 [Yersinia hibernica]
MTKTDYLMRLRKCTTIDTLERVIEKNKYELSDDELELFYSAADHRLAELTMNKLYDKIPPTVWQHVK

Nucleotide

• Length: 204 bp

>T111853 NZ_CP032487:2873306-2873509 [Yersinia hibernica]
ATGACAAAAACTGACTACCTGATGCGTTTAAGAAAATGTACGACTATTGATACGTTGGAGCGTGTAATTGAAAAAAATAA
GTATGAACTTTCAGATGATGAGTTGGAATTGTTTTACTCAGCAGCAGACCATCGCTTAGCTGAACTCACAATGAATAAAC
TTTATGATAAAATTCCACCTACTGTTTGGCAACATGTGAAGTAG

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 14234.98 Da
• Isoelectric Point: 4.4314

>AT111853 WP_025377437.1 NZ_CP032487:2872891-2873259 [Yersinia hibernica]
MDEYSPKRHDIAQLKFLCESLYDEGIATLGDSHHGWVNDPTSAVNLQLNDLIEHIASFVMSFKIKYPDDGDLSELVEEYL
DDTYTLFSSYGINDPELRRWQKTKERLFRLFSGEYICTLMKT

Nucleotide

• Length: 369 bp

>AT111853 NZ_CP032487:2872891-2873259 [Yersinia hibernica]
ATGGATGAGTACTCACCTAAGCGGCATGACATTGCCCAATTGAAGTTTCTATGTGAGAGTTTGTATGATGAAGGCATTGC
TACCCTTGGTGATAGTCACCACGGCTGGGTAAATGACCCAACTTCTGCCGTAAACTTACAACTTAATGATTTGATCGAGC
ATATAGCTTCATTTGTGATGAGTTTTAAAATAAAATACCCTGATGACGGTGACCTTTCGGAGTTAGTGGAAGAATATTTA
GACGATACATATACTCTGTTTAGTAGCTACGGAATCAATGATCCTGAATTGCGGCGCTGGCAAAAAACCAAAGAGCGTTT
ATTCAGACTTTTCTCAGGGGAATACATCTGTACCCTGATGAAAACTTAA

Structures

Toxin

• PDB: 2K5S