Detailed information of TA system TA111838

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2109950..2110479
• Replicon: chromosome
• Accession: NZ_CP032481
• Organism: Staphylococcus aureus strain O326

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: SaO326_RS10820
• Protein ID: WP_000621175.1
• Coordinates: 2109950..2110312 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: SaO326_RS10825
• Protein ID: WP_000948331.1
• Coordinates: 2110309..2110479 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SaO326_RS10795	2106929..2107699	-	771	WP_001041107.1	RNA polymerase sigma factor SigB	-
SaO326_RS10800	2107674..2108153	-	480	WP_001190825.1	anti-sigma B factor RsbW	-
SaO326_RS10805	2108155..2108481	-	327	WP_001052491.1	anti-sigma factor antagonist	-
SaO326_RS10810	2108600..2109601	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
SaO326_RS10820	2109950..2110312	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
SaO326_RS10825	2110309..2110479	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
SaO326_RS10830	2110564..2111712	-	1149	WP_001281146.1	alanine racemase	-
SaO326_RS10835	2111778..2112137	-	360	WP_000581198.1	holo-ACP synthase	-
SaO326_RS10840	2112141..2112632	-	492	WP_001205919.1	PH domain-containing protein	-
SaO326_RS10845	2112619..2114202	-	1584	WP_001294641.1	PH domain-containing protein	-
SaO326_RS10850	2114195..2114674	-	480	WP_001287083.1	hypothetical protein	-
SaO326_RS10855	2114883..2115443	-	561	WP_001092415.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T111838 WP_000621175.1 NZ_CP032481:c2110312-2109950 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T111838 NZ_CP032481:c2110312-2109950 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAATTGGATAAAGATTCAGTTATATTATTAGAACAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCATCAGAAAAATTAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT111838 WP_000948331.1 NZ_CP032481:c2110479-2110309 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT111838 NZ_CP032481:c2110479-2110309 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCTATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1