Detailed information of TA system TA111833

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TscAT/-
• Location: 421197..421735
• Replicon: chromosome
• Accession: NZ_CP032481
• Organism: Staphylococcus aureus strain O326

Toxin (Protein)

• Gene name: TscT
• Uniprot ID: -
• Locus tag: SaO326_RS02030
• Protein ID: WP_001103963.1
• Coordinates: 421409..421735 (+)
• Length: 109 a.a.

Antitoxin (Protein)

• Gene name: TscA
• Uniprot ID: -
• Locus tag: SaO326_RS02025
• Protein ID: WP_001058493.1
• Coordinates: 421197..421406 (+)
• Length: 70 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SaO326_RS01985	416598..416894	+	297	WP_001261460.1	30S ribosomal protein S6	-
SaO326_RS01990	416915..417418	+	504	WP_000934799.1	single-stranded DNA-binding protein	-
SaO326_RS01995	417470..417712	+	243	WP_000897044.1	30S ribosomal protein S18	-
SaO326_RS02000	417953..419266	-	1314	WP_120672824.1	site-specific integrase	-
SaO326_RS02005	419722..420417	-	696	WP_000668212.1	helix-turn-helix domain-containing protein	-
SaO326_RS02010	420561..420773	+	213	WP_000794656.1	helix-turn-helix transcriptional regulator	-
SaO326_RS02015	420774..421046	+	273	WP_000091734.1	helix-turn-helix domain-containing protein	-
SaO326_RS02020	421058..421204	+	147	WP_031824147.1	hypothetical protein	-
SaO326_RS02025	421197..421406	+	210	WP_001058493.1	hypothetical protein	Antitoxin
SaO326_RS02030	421409..421735	+	327	WP_001103963.1	DUF1474 family protein	Toxin
SaO326_RS02035	421800..422669	+	870	WP_001002688.1	primase alpha helix C-terminal domain-containing protein	-
SaO326_RS02040	422683..424392	+	1710	WP_000447458.1	DUF927 domain-containing protein	-
SaO326_RS02045	424704..425084	+	381	WP_000356937.1	hypothetical protein	-
SaO326_RS02050	425081..425722	+	642	WP_001019786.1	hypothetical protein	-
SaO326_RS02055	426069..426410	+	342	WP_001190600.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	416915..438618	21703

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Protein of unknown function DUF1474 (1-100)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 109 a.a.
• Molecular weight: 13092.50 Da
• Isoelectric Point: 4.7172

>T111833 WP_001103963.1 NZ_CP032481:421409-421735 [Staphylococcus aureus]
MNWEIKSLFSDLELLKDSFKDLKDNHGWHFDKLYPHEPNHVLNKDELIREGFSYHERRIHNNQMFDLFHLYIEQFDNIIE
KFYEIEKASSENFGEESDDAKNSIKVAE

Nucleotide

• Length: 327 bp

>T111833 NZ_CP032481:421409-421735 [Staphylococcus aureus]
ATGAATTGGGAAATTAAAAGTTTATTTAGCGATTTAGAGTTGTTGAAAGATAGTTTTAAGGATTTAAAGGATAATCATGG
TTGGCATTTTGATAAGTTATATCCTCATGAACCAAATCATGTCTTAAATAAAGATGAATTAATTAGAGAGGGTTTTTCTT
ATCATGAGAGACGTATTCACAATAATCAAATGTTTGATTTATTCCATCTCTATATAGAGCAGTTCGATAATATTATCGAA
AAGTTTTATGAAATAGAAAAAGCATCATCTGAGAACTTTGGCGAGGAATCAGATGACGCAAAGAATTCAATAAAAGTAGC
AGAGTAA

Antitoxin

Protein

• Length: 70 a.a.
• Molecular weight: 8152.19 Da
• Isoelectric Point: 4.4665

>AT111833 WP_001058493.1 NZ_CP032481:421197-421406 [Staphylococcus aureus]
MNNEQKEVIEHVVYQLELSVMNNLESYEHTEYVNGIEVVSEVSREKHLELIMKWCAQELKNNFQLGKGE

Nucleotide

• Length: 210 bp

>AT111833 NZ_CP032481:421197-421406 [Staphylococcus aureus]
ATGAATAATGAACAAAAAGAAGTAATAGAACACGTGGTTTATCAACTCGAGTTAAGTGTCATGAATAATTTGGAAAGTTA
TGAACACACAGAATATGTTAATGGTATTGAAGTGGTTTCAGAGGTTAGTCGTGAAAAGCACTTAGAATTGATAATGAAAT
GGTGCGCACAAGAATTAAAGAATAATTTTCAATTAGGAAAAGGAGAATAA