Detailed information of TA system TA111823

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): VapD-relB/-
• Location: 1083876..1084524
• Replicon: chromosome
• Accession: NZ_CP032477
• Organism: Helicobacter pylori strain 169-C-EK8

Toxin (Protein)

• Gene name: VapD
• Uniprot ID: T5D7N9
• Locus tag: D4K17_RS05280
• Protein ID: WP_000271050.1
• Coordinates: 1083876..1084160 (-)
• Length: 95 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: D4K17_RS05285
• Protein ID: WP_139522024.1
• Coordinates: 1084141..1084524 (-)
• Length: 128 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D4K17_RS05255	1080421..1081188	-	768	WP_000242349.1	ABC transporter ATP-binding protein	-
D4K17_RS05260	1081188..1082168	-	981	WP_139522022.1	iron ABC transporter permease	-
D4K17_RS05265	1082161..1083010	-	850	Protein_994	SDR family oxidoreductase	-
D4K17_RS05270	1083150..1083674	+	525	WP_001135623.1	acyl-CoA thioesterase	-
D4K17_RS05280	1083876..1084160	-	285	WP_000271050.1	endoribonuclease VapD	Toxin
D4K17_RS05285	1084141..1084524	-	384	WP_139522024.1	hypothetical protein	Antitoxin
D4K17_RS05290	1084895..1087096	-	2202	WP_139522025.1	outer membrane beta-barrel protein	-
D4K17_RS05305	1087423..1088057	+	635	Protein_999	hypothetical protein	-
D4K17_RS05310	1088008..1089120	-	1113	WP_139522026.1	hydrogenase formation protein HypD	-
D4K17_RS05315	1089138..1089374	-	237	WP_000335461.1	HypC/HybG/HupF family hydrogenase formation chaperone	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Virulence-associated protein D / CRISPR associated protein Cas2 (1-87)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 95 a.a.
• Molecular weight: 11188.74 Da
• Isoelectric Point: 4.8896

>T111823 WP_000271050.1 NZ_CP032477:c1084160-1083876 [Helicobacter pylori]
MYALAFDLKIEILKKEYGEPYNKAYDDLRQELELLGFEWTQGSVYVNYSKENTLAQVYKAINKLSQIEWFKKSVRDIRAF
KVEDFSDFTEIVKS

Nucleotide

• Length: 285 bp

>T111823 NZ_CP032477:c1084160-1083876 [Helicobacter pylori]
ATGTATGCTTTAGCGTTTGATTTAAAGATTGAGATTTTAAAAAAAGAATACGGAGAACCCTATAATAAAGCCTATGATGA
TTTAAGGCAAGAATTAGAGCTATTAGGGTTTGAATGGACTCAAGGGAGCGTTTATGTTAATTATTCTAAAGAAAACACTC
TAGCACAAGTTTATAAAGCGATCAATAAACTCTCTCAAATTGAGTGGTTTAAAAAGTCTGTTAGGGATATTAGAGCGTTT
AAGGTAGAGGACTTTAGCGATTTTACTGAGATTGTGAAATCCTAG

Antitoxin

Protein

• Length: 128 a.a.
• Molecular weight: 14998.15 Da
• Isoelectric Point: 8.6743

>AT111823 WP_139522024.1 NZ_CP032477:c1084524-1084141 [Helicobacter pylori]
MPNTTNKKDYTKYSQRQLFSLMHKKEQEIEKLKRDRAYLEKKMAKELEKKDKGFYNKLEELNKILQEINKRANDCCLGHE
IPNLQTQQAMRDALNGVNLTQIDSLDDLINELKRENSKGFENVCFSV

Nucleotide

• Length: 384 bp

>AT111823 NZ_CP032477:c1084524-1084141 [Helicobacter pylori]
ATGCCAAACACCACCAACAAAAAAGACTACACAAAATACAGCCAAAGACAGCTTTTTAGTCTCATGCATAAAAAAGAACA
AGAAATTGAAAAGCTAAAAAGGGATAGGGCGTATCTTGAAAAGAAAATGGCTAAAGAGCTGGAGAAAAAAGATAAAGGTT
TTTATAACAAATTAGAAGAGTTGAACAAAATCTTACAAGAGATCAACAAAAGAGCTAATGATTGCTGTTTGGGGCATGAG
ATCCCAAACCTTCAAACGCAACAAGCCATGAGAGATGCGTTAAATGGGGTTAATCTCACTCAAATTGATAGTTTAGATGA
TCTTATAAACGAGTTAAAAAGAGAAAATAGTAAAGGTTTTGAAAATGTATGCTTTAGCGTTTGA

Structures

Toxin

• PDB: 3UI3