Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/RelE(toxin)
Location 56359..56828 Replicon plasmid unnamed2
Accession NZ_CP032458
Organism Pseudomonas savastanoi strain CAS03

Toxin (Protein)


Gene name relE Uniprot ID Q48BA0
Locus tag D5S10_RS28505 Protein ID WP_002556029.1
Coordinates 56547..56828 (+) Length 94 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID Q48BA1
Locus tag D5S10_RS28500 Protein ID WP_002556028.1
Coordinates 56359..56547 (+) Length 63 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
D5S10_RS28465 51441..51968 - 528 WP_192485863.1 transcriptional regulator -
D5S10_RS28470 52232..52582 + 351 WP_004666693.1 helix-turn-helix transcriptional regulator -
D5S10_RS28475 52670..53140 + 471 WP_192485864.1 bacteriocin immunity protein -
D5S10_RS28480 53386..53766 + 381 WP_074929343.1 hypothetical protein -
D5S10_RS28485 54301..54555 - 255 WP_003381801.1 hypothetical protein -
D5S10_RS28490 54591..55223 - 633 WP_003381804.1 ParA family protein -
D5S10_RS28495 55546..56181 + 636 WP_054995158.1 recombinase family protein -
D5S10_RS28500 56359..56547 + 189 WP_002556028.1 hypothetical protein Antitoxin
D5S10_RS28505 56547..56828 + 282 WP_002556029.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
D5S10_RS28510 56980..58527 - 1548 WP_054076397.1 glycoside hydrolase family 28 protein -
D5S10_RS28515 58611..58834 - 224 Protein_69 carbon storage regulator -
D5S10_RS28520 59403..59673 + 271 Protein_70 transposase -
D5S10_RS28525 59807..60787 - 981 WP_054078085.1 IS5-like element ISPsy2 family transposase -
D5S10_RS28530 60861..61361 + 501 WP_019740153.1 type VI secretion system tube protein Hcp -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..73281 73281


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(4-90)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 94 a.a.        Molecular weight: 10675.35 Da        Isoelectric Point: 8.4392

>T111811 WP_002556029.1 NZ_CP032458:56547-56828 [Pseudomonas savastanoi]
MLPVVWLSPALDDLREIATYIAWENPSAARRLKSLLQEAIEPVAEHPYLYRSGRAPGTRELVAHPNYVLVYRVTLKRIEV
VNVIHARQEYPSP

Download         Length: 282 bp

>T111811 NZ_CP032458:56547-56828 [Pseudomonas savastanoi]
ATGCTGCCGGTGGTCTGGCTCAGTCCCGCACTGGATGATCTGCGCGAAATCGCAACCTACATCGCATGGGAAAACCCGAG
CGCAGCGCGCCGGTTGAAAAGCCTCCTGCAGGAGGCTATCGAGCCGGTAGCCGAACACCCCTATTTATATCGTTCTGGGC
GAGCGCCAGGCACTCGCGAGCTGGTGGCTCACCCAAACTACGTCTTGGTCTATCGTGTCACCCTCAAGCGCATCGAGGTC
GTGAACGTAATCCACGCTAGGCAGGAATACCCCTCTCCCTGA

Antitoxin


Download         Length: 63 a.a.        Molecular weight: 7043.86 Da        Isoelectric Point: 4.2550

>AT111811 WP_002556028.1 NZ_CP032458:56359-56547 [Pseudomonas savastanoi]
MANLSPIVSEFETDEQAASYDRWFRAKVAASLADPRPAIPHDEVMAEIEALIDQVETQREKR

Download         Length: 189 bp

>AT111811 NZ_CP032458:56359-56547 [Pseudomonas savastanoi]
ATGGCCAACCTCTCACCCATCGTTTCAGAATTCGAAACCGATGAACAGGCCGCAAGCTACGACCGCTGGTTTCGCGCCAA
GGTCGCCGCCAGTCTGGCCGATCCGCGTCCGGCCATCCCTCACGATGAGGTCATGGCGGAAATAGAAGCGCTGATCGATC
AGGTTGAAACCCAACGTGAGAAACGCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M9HVA1

Antitoxin

Source ID Structure
AlphaFold DB A0A2G9KWB9

References