Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 5915238..5915757 Replicon chromosome
Accession NZ_CP032456
Organism Pseudomonas savastanoi strain CAS03

Toxin (Protein)


Gene name relE Uniprot ID Q48Q48
Locus tag D5S10_RS26540 Protein ID WP_002551445.1
Coordinates 5915238..5915528 (-) Length 97 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID A0A0P9WSS8
Locus tag D5S10_RS26545 Protein ID WP_016982665.1
Coordinates 5915518..5915757 (-) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
D5S10_RS26520 5910841..5911821 + 981 WP_057434159.1 IS5-like element ISPsy2 family transposase -
D5S10_RS26525 5912009..5912758 + 750 WP_192485548.1 hypothetical protein -
D5S10_RS26530 5913374..5913988 - 615 WP_016981235.1 histidine phosphatase family protein -
D5S10_RS26535 5914268..5915005 + 738 WP_005746662.1 hypothetical protein -
D5S10_RS26540 5915238..5915528 - 291 WP_002551445.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
D5S10_RS26545 5915518..5915757 - 240 WP_016982665.1 type II toxin-antitoxin system RelB/DinJ family antitoxin Antitoxin
D5S10_RS26550 5915921..5916394 - 474 WP_016982664.1 GNAT family N-acetyltransferase -
D5S10_RS26555 5916496..5917044 - 549 WP_016982663.1 GNAT family N-acetyltransferase -
D5S10_RS26560 5917204..5917698 + 495 WP_004667254.1 hypothetical protein -
D5S10_RS26565 5917732..5919006 - 1275 WP_016982662.1 OprD family porin -
D5S10_RS26570 5919221..5919442 - 222 WP_162267814.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 5910838..5911821 983


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-83)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-70)


Sequences

Toxin        


Download         Length: 97 a.a.        Molecular weight: 11316.19 Da        Isoelectric Point: 10.2579

>T111810 WP_002551445.1 NZ_CP032456:c5915528-5915238 [Pseudomonas savastanoi]
MTYNLEFDARALKEWHKLGDTVRQQLKKKLATILVAPRVEANRLHALPDCYKIKLRSSGYRLVYQVIDQEVVVFVVAVDK
REREEVYRKATDRLGR

Download         Length: 291 bp

>T111810 NZ_CP032456:c5915528-5915238 [Pseudomonas savastanoi]
ATGACCTATAACCTCGAATTCGATGCGCGGGCTCTAAAGGAATGGCACAAATTAGGAGATACCGTGCGTCAGCAGCTCAA
GAAAAAGCTGGCTACGATTCTGGTTGCTCCGCGTGTTGAAGCCAATCGTCTGCATGCATTGCCTGACTGCTACAAAATCA
AACTGCGCAGTAGCGGCTATCGGCTGGTTTATCAGGTTATTGACCAAGAGGTTGTGGTCTTCGTGGTTGCGGTAGACAAG
CGTGAACGGGAAGAGGTTTATCGCAAGGCGACGGACCGGCTGGGTAGGTAA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 8985.37 Da        Isoelectric Point: 4.6017

>AT111810 WP_016982665.1 NZ_CP032456:c5915757-5915518 [Pseudomonas savastanoi]
MASINVRIDDDLKARAYLELEKLGVTPSELLRQTLQYVAERGQLPFKTVLMTEEDEALLATVRERLATPQRVKVSLDDL

Download         Length: 240 bp

>AT111810 NZ_CP032456:c5915757-5915518 [Pseudomonas savastanoi]
ATGGCGTCTATCAACGTTCGTATCGACGACGATCTCAAAGCGCGTGCTTACCTCGAGCTGGAAAAGCTTGGCGTCACCCC
CTCGGAGTTGCTGCGCCAAACCCTGCAGTACGTAGCTGAGCGTGGTCAGCTACCCTTCAAGACGGTTCTGATGACAGAGG
AAGACGAAGCGCTGTTGGCAACTGTGCGTGAGCGCCTTGCAACTCCGCAGCGGGTCAAGGTTTCACTGGATGACCTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2G4DBS4

Antitoxin

Source ID Structure
AlphaFold DB A0A0P9WSS8

References