Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/upstrm_HI1419-dnstrm_HI1420
Location 5340409..5341004 Replicon chromosome
Accession NZ_CP032456
Organism Pseudomonas savastanoi strain CAS03

Toxin (Protein)


Gene name relE Uniprot ID A0A0Q0BQQ0
Locus tag D5S10_RS23795 Protein ID WP_005782087.1
Coordinates 5340409..5340711 (+) Length 101 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID A0A0P9S822
Locus tag D5S10_RS23800 Protein ID WP_005746689.1
Coordinates 5340714..5341004 (+) Length 97 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
D5S10_RS23775 5335742..5337082 - 1341 WP_016982358.1 precorrin-3B synthase -
D5S10_RS23780 5337208..5338410 + 1203 WP_016982357.1 bifunctional cobalt-precorrin-7 (C(5))-methyltransferase/cobalt-precorrin-6B (C(15))-methyltransferase -
D5S10_RS23785 5338410..5339522 + 1113 WP_004655757.1 cobalt-precorrin-5B (C(1))-methyltransferase -
D5S10_RS23790 5339519..5340259 + 741 WP_016982356.1 cobalt-precorrin-6A reductase -
D5S10_RS23795 5340409..5340711 + 303 WP_005782087.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
D5S10_RS23800 5340714..5341004 + 291 WP_005746689.1 putative addiction module antidote protein Antitoxin
D5S10_RS23805 5341075..5341536 + 462 WP_002555389.1 copper chaperone PCu(A)C -
D5S10_RS23810 5341550..5341984 + 435 WP_016982355.1 DUF2946 domain-containing protein -
D5S10_RS23815 5342069..5343442 + 1374 WP_005746685.1 PepSY domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-86)

Antitoxin

Uncharacterized protein HI1420

(4-95)


Sequences

Toxin        


Download         Length: 101 a.a.        Molecular weight: 11507.24 Da        Isoelectric Point: 10.6008

>T111808 WP_005782087.1 NZ_CP032456:5340409-5340711 [Pseudomonas savastanoi]
MNRFEQTPAFAEWLRSLKDSIGRARILARIRAAELGNFGDCDALGQGVRELRIHHGPGYRVYFTRRTGVVYLLLIAGDKS
SQKRDIKFARQLARELCERE

Download         Length: 303 bp

>T111808 NZ_CP032456:5340409-5340711 [Pseudomonas savastanoi]
ATGAATCGCTTTGAACAGACGCCCGCTTTTGCGGAGTGGTTAAGGTCGTTGAAGGACTCCATTGGCAGAGCTCGAATTCT
GGCTCGAATAAGAGCAGCAGAGCTCGGTAACTTCGGGGACTGCGATGCTTTAGGTCAAGGCGTCAGAGAGCTACGAATAC
ACCATGGACCAGGTTATCGGGTGTATTTTACCCGACGCACGGGTGTGGTTTACCTGCTCTTGATCGCTGGTGACAAATCG
TCTCAAAAGCGCGACATAAAATTTGCCAGACAGCTGGCGAGAGAGCTCTGTGAGAGGGAGTAA

Antitoxin


Download         Length: 97 a.a.        Molecular weight: 10500.87 Da        Isoelectric Point: 4.5720

>AT111808 WP_005746689.1 NZ_CP032456:5340714-5341004 [Pseudomonas savastanoi]
MNNETTPFDTVDYLKTPEDMANYLDACLEEDSGDGLLVRAALNDIARAYGMTQIARDTGLGRESLYKALGANGNPEFTTI
MKVVRAMGIKLHASTR

Download         Length: 291 bp

>AT111808 NZ_CP032456:5340714-5341004 [Pseudomonas savastanoi]
ATGAACAACGAGACGACACCGTTCGATACCGTCGACTATTTGAAGACACCTGAAGATATGGCCAATTATCTGGATGCGTG
TCTTGAAGAGGATTCAGGAGACGGGCTGCTCGTAAGGGCCGCGCTGAACGACATAGCAAGGGCTTATGGCATGACACAAA
TCGCACGTGATACAGGTCTTGGTCGTGAGAGCCTCTACAAAGCGCTGGGTGCAAACGGTAATCCAGAGTTCACGACCATC
ATGAAAGTCGTGAGGGCGATGGGGATCAAACTACACGCAAGTACGCGCTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0Q0BQQ0

Antitoxin

Source ID Structure
AlphaFold DB A0A0P9S822

References