Detailed information of TA system TA111794

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Fic(toxin)
• Location: 3256645..3257232
• Replicon: chromosome
• Accession: NZ_CP032427
• Organism: Streptomyces griseorubiginosus strain 3E-1

Toxin (Protein)

• Gene name: doc
• Uniprot ID: -
• Locus tag: DWG14_RS14320
• Protein ID: WP_120054314.1
• Coordinates: 3256855..3257232 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: A0A385ZHJ3
• Locus tag: DWG14_RS14315
• Protein ID: WP_019073218.1
• Coordinates: 3256645..3256842 (+)
• Length: 66 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DWG14_RS14295	3252405..3252878	+	474	WP_120050981.1	NUDIX domain-containing protein	-
DWG14_RS14300	3252866..3253435	-	570	WP_174856901.1	HD domain-containing protein	-
DWG14_RS14305	3253447..3253893	-	447	WP_120050983.1	GntR family transcriptional regulator	-
DWG14_RS43465	3254198..3254761	-	564	WP_174857046.1	DUF4231 domain-containing protein	-
DWG14_RS14315	3256645..3256842	+	198	WP_019073218.1	hypothetical protein	Antitoxin
DWG14_RS14320	3256855..3257232	+	378	WP_120054314.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
DWG14_RS14325	3257510..3258112	+	603	WP_162952021.1	hypothetical protein	-
DWG14_RS43020	3258396..3258953	+	558	WP_162952022.1	hypothetical protein	-
DWG14_RS14330	3259139..3259993	+	855	WP_120050986.1	sel1 repeat family protein	-
DWG14_RS43025	3260824..3261123	+	300	WP_162952023.1	hypothetical protein	-
DWG14_RS14340	3261428..3262144	-	717	WP_120050988.1	SDR family oxidoreductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Death on curing protein (3-122)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13431.08 Da
• Isoelectric Point: 4.2897

>T111794 WP_120054314.1 NZ_CP032427:3256855-3257232 [Streptomyces griseorubiginosus]
ITEYLDANDVIQIAEITLGGRVGVRDWGLLQSAVARPQTSVFGEDAYPTLFEKAAALLHSVSSNHSLVDGNKRTGWASAI
VFLDINGHPLKEPLDEDAAEEFVLAVAQSQLSNLEIAGRLQAFVA

Nucleotide

• Length: 378 bp

>T111794 NZ_CP032427:3256855-3257232 [Streptomyces griseorubiginosus]
ATAACCGAGTACCTGGACGCCAACGACGTCATCCAGATCGCGGAGATTACTCTTGGTGGCCGTGTTGGCGTACGTGACTG
GGGGCTGCTGCAAAGCGCCGTCGCTCGCCCTCAAACCAGCGTTTTTGGTGAGGATGCCTACCCAACGCTCTTCGAAAAGG
CAGCAGCTCTGCTGCACTCCGTCTCCAGCAATCACAGCCTGGTAGACGGGAACAAGCGCACCGGGTGGGCATCTGCCATC
GTCTTCCTAGACATCAACGGACATCCGCTGAAGGAACCTCTGGATGAGGACGCAGCTGAGGAGTTCGTGTTGGCAGTAGC
TCAGTCTCAGTTGTCGAACCTGGAGATCGCTGGCCGCTTGCAAGCATTCGTTGCTTAG

Antitoxin

Protein

• Length: 66 a.a.
• Molecular weight: 7061.17 Da
• Isoelectric Point: 10.5972

>AT111794 WP_019073218.1 NZ_CP032427:3256645-3256842 [Streptomyces griseorubiginosus]
MPALNVTFTDDEMATLRDQAAKEDVSMKALAHDAVLAEVHRRKVTAAAIRTARISAGLNKRLATK

Nucleotide

• Length: 198 bp

>AT111794 NZ_CP032427:3256645-3256842 [Streptomyces griseorubiginosus]
ATGCCTGCACTGAACGTCACTTTCACTGACGACGAGATGGCCACACTCCGCGACCAGGCAGCAAAGGAGGATGTCAGCAT
GAAGGCACTGGCGCACGACGCCGTCTTGGCTGAGGTCCACCGGCGCAAGGTGACGGCTGCCGCCATCAGGACTGCCCGTA
TCTCCGCAGGCCTGAACAAGCGCCTCGCCACCAAGTAA

Structures

Antitoxin

• AlphaFold DB: A0A385ZHJ3