Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 64807..65332 Replicon plasmid pOXA1_020001
Accession NZ_CP032425
Organism Escherichia coli strain SCEC020001

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag C7V21_RS00820 Protein ID WP_001159871.1
Coordinates 64807..65112 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID H9TJP1
Locus tag C7V21_RS00825 Protein ID WP_000813630.1
Coordinates 65114..65332 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C7V21_RS00805 60769..61935 - 1167 WP_000772446.1 plasmid-partitioning protein SopA -
C7V21_RS00810 62523..63278 - 756 WP_000852146.1 replication initiation protein RepE -
C7V21_RS00815 64000..64806 - 807 WP_000016970.1 site-specific integrase -
C7V21_RS00820 64807..65112 - 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
C7V21_RS00825 65114..65332 - 219 WP_000813630.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
C7V21_RS00835 65892..66122 + 231 WP_001261286.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
C7V21_RS00840 66119..66535 + 417 WP_001034044.1 type II toxin-antitoxin system VapC family toxin -
C7V21_RS00845 66610..68175 + 1566 WP_001128474.1 AAA family ATPase -
C7V21_RS00850 68160..69182 + 1023 WP_000361402.1 helicase UvrD -
C7V21_RS00855 69436..70133 - 698 Protein_84 IS1-like element IS1A family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid blaOXA-1 / aac(6')-Ib-cr / tet(A) / sitABCD iutA / iucD / iucC / iucB / iucA 1..88392 88392


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T111771 WP_001159871.1 NZ_CP032425:c65112-64807 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T111771 NZ_CP032425:c65112-64807 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTCCAGAGTGACATTATTGACAC
GCCTGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGTTGTCAGATAAGGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAGGAAGTG
GCTGATCTCAGCCACCGAGAAAATGACATCAAAAATGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8354.27 Da        Isoelectric Point: 4.5203

>AT111771 WP_000813630.1 NZ_CP032425:c65332-65114 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTLQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT111771 NZ_CP032425:c65332-65114 [Escherichia coli]
ATGAAGCAGCGCATTACAGTCACCGTCGACAGTGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGGCT
GGTAAGCACCACCCTGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGCAGGCAGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCCGACGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
AlphaFold DB A0A829CEF5

References