Detailed information of TA system TA111764

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazF-pemI/MazF(toxin)
• Location: 2164860..2165490
• Replicon: chromosome
• Accession: NZ_CP032418
• Organism: Paenisporosarcina sp. K2R23-3

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: D3873_RS11155
• Protein ID: WP_119884080.1
• Coordinates: 2164860..2165210 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: -
• Locus tag: D3873_RS11160
• Protein ID: WP_205536264.1
• Coordinates: 2165215..2165490 (-)
• Length: 92 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D3873_RS11120	2160395..2161177	-	783	WP_119884073.1	RNA polymerase sigma factor SigB	-
D3873_RS11125	2161155..2161625	-	471	WP_119884074.1	anti-sigma B factor RsbW	-
D3873_RS11130	2161625..2161957	-	333	WP_119884075.1	STAS domain-containing protein	-
D3873_RS11135	2162039..2163043	-	1005	WP_119884076.1	PP2C family protein-serine/threonine phosphatase	-
D3873_RS11140	2163056..2163457	-	402	WP_119884077.1	anti-sigma regulatory factor	-
D3873_RS11145	2163460..2163858	-	399	WP_205536263.1	STAS domain-containing protein	-
D3873_RS11150	2163819..2164649	-	831	WP_119884079.1	STAS domain-containing protein	-
D3873_RS11155	2164860..2165210	-	351	WP_119884080.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
D3873_RS11160	2165215..2165490	-	276	WP_205536264.1	transcriptional regulator	Antitoxin
D3873_RS11165	2165613..2166722	-	1110	WP_119884082.1	alanine racemase	-
D3873_RS11170	2166747..2167730	-	984	WP_205536300.1	outer membrane lipoprotein carrier protein LolA	-
D3873_RS11175	2167810..2168163	-	354	WP_119884084.1	holo-ACP synthase	-
D3873_RS11180	2168231..2168857	+	627	WP_119884085.1	rhomboid family intramembrane serine protease	-
D3873_RS11185	2168925..2170394	-	1470	WP_205536265.1	PH domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-113)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12910.91 Da
• Isoelectric Point: 4.9057

>T111764 WP_119884080.1 NZ_CP032418:c2165210-2164860 [Paenisporosarcina sp. K2R23-3]
MNVKRGDVFFADLSPVIGSEQGGTRPVLVIQNDIGNRFSPTVIIAAITAQIQKAKLPTHVEIDAKKYGFERDSVILLEQL
RTIDKSRLTDKITQLDDEVMEKVDEALEISIGLVKF

Nucleotide

• Length: 351 bp

>T111764 NZ_CP032418:c2165210-2164860 [Paenisporosarcina sp. K2R23-3]
TTGAACGTTAAACGAGGAGACGTCTTTTTTGCAGATTTGTCACCAGTGATCGGGTCAGAGCAAGGTGGTACGAGGCCCGT
TCTGGTGATTCAAAATGATATTGGCAATCGATTTAGTCCGACGGTGATCATTGCTGCTATCACAGCGCAAATTCAAAAAG
CAAAGTTGCCAACACATGTAGAGATAGATGCAAAAAAGTATGGCTTTGAACGGGATTCCGTCATACTGTTGGAACAATTG
CGAACGATTGATAAATCACGTCTAACAGACAAAATCACACAATTAGACGATGAAGTGATGGAAAAAGTAGATGAAGCGTT
AGAAATTAGCATCGGCTTAGTCAAATTTTAA

Antitoxin

Protein

• Length: 92 a.a.
• Molecular weight: 10506.92 Da
• Isoelectric Point: 6.7259

>AT111764 WP_205536264.1 NZ_CP032418:c2165490-2165215 [Paenisporosarcina sp. K2R23-3]
VNKNRKTKEVIVRLPRNYATDISSLNNAVSQEEYVYISTKKVINQVEPNLIREAMMRGYVEMSHINLTLASESLHLEYEA
EHTWERLVSGG

Nucleotide

• Length: 276 bp

>AT111764 NZ_CP032418:c2165490-2165215 [Paenisporosarcina sp. K2R23-3]
GTGAATAAAAACAGAAAAACAAAGGAAGTCATTGTGCGTCTTCCAAGAAACTATGCAACGGATATTAGCTCATTGAACAA
CGCCGTGAGCCAAGAAGAGTATGTTTATATTTCGACAAAAAAGGTAATTAATCAAGTGGAACCAAATCTTATACGAGAAG
CGATGATGCGTGGATACGTAGAAATGTCCCACATCAATTTAACGCTTGCAAGTGAAAGCTTGCACTTAGAGTATGAAGCA
GAGCATACGTGGGAGCGTCTCGTAAGCGGAGGATGA