Detailed information of TA system TA111758

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazF-pemI/MazF(toxin)
• Location: 740862..741488
• Replicon: chromosome
• Accession: NZ_CP032412
• Organism: Paenibacillus lautus strain E7593-69

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: W4ARZ2
• Locus tag: D5F53_RS03675
• Protein ID: WP_006212388.1
• Coordinates: 741138..741488 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: -
• Locus tag: D5F53_RS03670
• Protein ID: WP_006212389.1
• Coordinates: 740862..741134 (+)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D5F53_RS03650	735896..736396	+	501	WP_119846577.1	winged helix-turn-helix domain-containing protein	-
D5F53_RS03655	736460..737788	+	1329	WP_119846578.1	MFS transporter	-
D5F53_RS03660	738052..739164	+	1113	WP_119846579.1	outer membrane lipoprotein carrier protein LolA	-
D5F53_RS03665	739437..740624	+	1188	WP_119846580.1	alanine racemase	-
D5F53_RS03670	740862..741134	+	273	WP_006212389.1	ribbon-helix-helix protein, CopG family	Antitoxin
D5F53_RS03675	741138..741488	+	351	WP_006212388.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
D5F53_RS03680	741929..744124	+	2196	WP_208652615.1	helix-hairpin-helix domain-containing protein	-
D5F53_RS03685	744233..744673	-	441	WP_119846582.1	GNAT family N-acetyltransferase	-
D5F53_RS03690	744739..745299	-	561	WP_119846583.1	CGNR zinc finger domain-containing protein	-
D5F53_RS03695	745445..745915	+	471	WP_119846584.1	DinB family protein	-
D5F53_RS03700	746014..746145	-	132	WP_015737250.1	cortex morphogenetic protein CmpA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (2-113)

Antitoxin

• Ribbon-helix-helix protein, CopG (6-43)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12751.64 Da
• Isoelectric Point: 4.8265

>T111758 WP_006212388.1 NZ_CP032412:741138-741488 [Paenibacillus lautus]
VIVKRGDVFFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAASHGFDRDSVILLEQI
RTIDKQRLTDKITHLDDEMMSKVDDSLQISLGLIDF

Nucleotide

• Length: 351 bp

>T111758 NZ_CP032412:741138-741488 [Paenibacillus lautus]
GTGATCGTTAAACGGGGCGACGTATTTTTTGCGGATTTATCACCTGTTGTCGGTTCTGAGCAAGGCGGCGTGAGACCGGT
CCTTGTCATTCAGAACGATATCGGCAACCGGTTTAGTCCGACTGTGATTGTTGCAGCCATTACGGCACAGATTCAAAAAG
CCAAGCTGCCGACTCACGTCGAAATCGACGCGGCGTCGCATGGATTTGACCGGGATTCCGTCATTTTGCTAGAGCAGATC
CGGACGATTGACAAGCAGCGGCTAACCGACAAAATCACCCATCTTGATGATGAAATGATGAGCAAGGTGGATGATTCCCT
GCAAATAAGTCTGGGCTTGATTGATTTTTGA

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10500.04 Da
• Isoelectric Point: 6.4708

>AT111758 WP_006212389.1 NZ_CP032412:740862-741134 [Paenibacillus lautus]
MQNTKRIMISLPDHLLQEVDGIAQLENSNRSELIRQAMKLYLNERKKRYIRESMQRGYMEMAKINLTMASEAFHAEEDAD
NTLDRLVSGV

Nucleotide

• Length: 273 bp

>AT111758 NZ_CP032412:740862-741134 [Paenibacillus lautus]
ATGCAGAACACCAAACGAATCATGATCAGTTTACCGGATCATCTTTTGCAGGAAGTGGACGGGATCGCTCAGCTGGAAAA
TTCCAACCGCAGTGAGCTTATTAGGCAGGCCATGAAGTTGTACCTGAATGAACGGAAGAAACGTTACATTCGTGAATCCA
TGCAGCGGGGTTATATGGAAATGGCCAAAATTAACCTGACCATGGCTTCTGAGGCGTTTCACGCGGAGGAAGATGCAGAC
AACACTCTAGACCGCTTAGTAAGCGGGGTGTAG

Structures

Toxin

• AlphaFold DB: A0A1S2EZN8