Detailed information of TA system TA111757

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 4013787..4014419
• Replicon: chromosome
• Accession: NZ_CP032410
• Organism: Brevibacillus laterosporus strain E7593-50

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: A0A0F7EEZ8
• Locus tag: D5F52_RS18655
• Protein ID: WP_003333534.1
• Coordinates: 4013787..4014137 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: D5F52_RS18660
• Protein ID: WP_173645602.1
• Coordinates: 4014141..4014419 (-)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D5F52_RS18625	4008929..4009642	-	714	WP_119734124.1	tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex dimerization subunit type 1 TsaB	-
D5F52_RS18630	4009623..4010093	-	471	WP_119734125.1	tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex ATPase subunit type 1 TsaE	-
D5F52_RS18635	4010159..4010623	-	465	WP_018673801.1	SprT family protein	-
D5F52_RS18640	4010744..4011079	+	336	WP_018673802.1	hypothetical protein	-
D5F52_RS18645	4011195..4011320	+	126	WP_003333532.1	cortex morphogenetic protein CmpA	-
D5F52_RS18650	4011398..4013557	-	2160	WP_119734126.1	RNA-binding transcriptional accessory protein	-
D5F52_RS18655	4013787..4014137	-	351	WP_003333534.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
D5F52_RS18660	4014141..4014419	-	279	WP_173645602.1	ribbon-helix-helix protein, CopG family	Antitoxin
D5F52_RS18665	4014583..4015788	-	1206	WP_018673804.1	alanine racemase	-
D5F52_RS18670	4015792..4016235	-	444	WP_018673805.1	hypothetical protein	-
D5F52_RS18675	4016468..4017499	-	1032	WP_018673806.1	DUF4367 domain-containing protein	-
D5F52_RS18680	4017847..4018179	-	333	WP_018673807.1	gamma-type small acid-soluble spore protein	-
D5F52_RS18685	4018388..4018720	-	333	WP_018673808.1	metalloregulator ArsR/SmtB family transcription factor	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-113)

Antitoxin

• Ribbon-helix-helix protein, CopG (8-45)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12932.83 Da
• Isoelectric Point: 4.8553

>T111757 WP_003333534.1 NZ_CP032410:c4014137-4013787 [Brevibacillus laterosporus]
VNVKRGDVFFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKTYGFDRDSVILLEQI
RTIDKQRLTDKITHLDDEMMERVNESLQISLGLIDF

Nucleotide

• Length: 351 bp

>T111757 NZ_CP032410:c4014137-4013787 [Brevibacillus laterosporus]
GTGAATGTTAAACGTGGCGATGTATTTTTCGCGGACCTTTCCCCTGTGGTTGGCTCCGAGCAAGGTGGAGTTAGACCGGT
ATTAGTTATACAAAACGATATTGGAAATCGATTTAGTCCGACGGTTATCGTCGCTGCGATCACTGCTCAGATTCAAAAGG
CAAAGCTGCCTACGCATGTGGAAATCGATGCAAAAACATATGGGTTTGATCGTGACTCTGTCATCTTATTGGAACAAATA
AGAACCATTGATAAGCAAAGGTTGACTGATAAAATCACTCATCTAGACGATGAGATGATGGAAAGAGTGAATGAATCACT
TCAGATCAGCCTCGGATTAATAGATTTTTAA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10729.43 Da
• Isoelectric Point: 9.6543

>AT111757 WP_173645602.1 NZ_CP032410:c4014419-4014141 [Brevibacillus laterosporus]
MSKSNTKRIMISLPQHLLQEVDGVVQKERSNRSELIRQAMSLYLKERKKRFIRETMQRGYLEMAKINLNMASEAFEAEEE
ADLTLDRLVSGV

Nucleotide

• Length: 279 bp

>AT111757 NZ_CP032410:c4014419-4014141 [Brevibacillus laterosporus]
TTGTCTAAAAGCAATACAAAACGTATCATGATCAGTTTGCCGCAACATTTATTGCAAGAGGTAGACGGAGTCGTGCAAAA
AGAGAGATCAAACCGAAGTGAATTGATTCGTCAAGCGATGAGTCTCTACCTCAAGGAGCGAAAAAAACGATTTATACGAG
AAACAATGCAACGTGGCTATTTGGAAATGGCTAAAATCAATCTCAATATGGCATCGGAGGCTTTTGAAGCAGAAGAGGAA
GCCGATCTTACCTTAGACCGCTTAGTTAGCGGGGTGTAG

Structures

Toxin

• AlphaFold DB: A0A0F7EEZ8