Detailed information of TA system TA111755

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 2451..3082
• Replicon: plasmid p1
• Accession: NZ_CP032406
• Organism: Rhizobium rosettiformans strain MAE2-X

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: D4A92_RS21600
• Protein ID: WP_203020489.1
• Coordinates: 2451..2852 (-)
• Length: 134 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: D4A92_RS21605
• Protein ID: WP_203020491.1
• Coordinates: 2852..3082 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D4A92_RS21600	2451..2852	-	402	WP_203020489.1	tRNA(fMet)-specific endonuclease VapC	Toxin
D4A92_RS21605	2852..3082	-	231	WP_203020491.1	antitoxin	Antitoxin
D4A92_RS21610	4004..5050	-	1047	Protein_3	IS5 family transposase	-
D4A92_RS21615	5338..7113	-	1776	WP_203020493.1	ABC transporter ATP-binding protein	-
D4A92_RS21620	7110..8069	-	960	WP_203020495.1	acetamidase/formamidase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	adeG	1..510647	510647

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-124)

Antitoxin

• SpoVT-AbrB domain (7-53)

Sequences

Toxin

Protein

• Length: 134 a.a.
• Molecular weight: 15032.17 Da
• Isoelectric Point: 7.2438

>T111755 WP_203020489.1 NZ_CP032406:c2852-2451 [Rhizobium rosettiformans]
MLKYMLDTNICIFTIKNRPQQVREAFNRFHDQLCISSVSLMELIYGAEKSASPEKNLPVVEGFAARLEVLPYDEQAANHT
GQLRAELARSGTPIGPYDQLIAGHARSRGLIMVTNNRREFDRVPGLRVEDWTG

Nucleotide

• Length: 402 bp

>T111755 NZ_CP032406:c2852-2451 [Rhizobium rosettiformans]
ATGCTGAAGTACATGCTGGACACCAACATTTGCATTTTCACGATCAAAAATCGGCCACAGCAGGTGCGCGAGGCATTCAA
TCGTTTCCATGACCAATTGTGCATCAGCTCGGTCAGTTTGATGGAATTGATTTACGGAGCGGAAAAGTCAGCCAGTCCTG
AAAAGAACCTCCCCGTTGTGGAGGGCTTCGCGGCGCGCCTTGAAGTGTTGCCTTACGATGAACAGGCGGCAAATCATACG
GGTCAATTGCGGGCCGAACTGGCACGCAGTGGAACTCCGATCGGTCCATATGATCAATTGATTGCTGGACATGCTCGCTC
GCGTGGGCTGATTATGGTGACGAACAACCGAAGGGAATTTGACCGCGTTCCGGGCCTTCGGGTGGAAGATTGGACGGGTT
AG

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8448.43 Da
• Isoelectric Point: 4.3867

>AT111755 WP_203020491.1 NZ_CP032406:c3082-2852 [Rhizobium rosettiformans]
VEQGAVFQSNRSQAIRLPKAVALPDDVKRVDIVIVGRTRIIAPAGEIWDSWFEGEAATTDFMSERDQAASQEREAF

Nucleotide

• Length: 231 bp

>AT111755 NZ_CP032406:c3082-2852 [Rhizobium rosettiformans]
GTGGAGCAAGGAGCAGTATTCCAGAGCAATCGAAGCCAGGCTATCCGTCTTCCAAAGGCAGTAGCTTTGCCTGATGATGT
AAAGCGCGTCGATATTGTAATCGTCGGACGTACTCGCATTATCGCGCCTGCTGGTGAGATCTGGGACAGCTGGTTCGAAG
GGGAAGCGGCTACAACTGACTTCATGAGTGAACGTGATCAAGCCGCCTCCCAGGAGCGCGAGGCATTTTAA