Detailed information of TA system TA111754

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE(toxin)
• Location: 4234031..4234701
• Replicon: chromosome
• Accession: NZ_CP032405
• Organism: Rhizobium rosettiformans strain MAE2-X

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: D4A92_RS20835
• Protein ID: WP_203017041.1
• Coordinates: 4234441..4234701 (-)
• Length: 87 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: D4A92_RS20830
• Protein ID: WP_203017040.1
• Coordinates: 4234031..4234441 (-)
• Length: 137 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D4A92_RS20810	4230216..4230680	+	465	WP_054148481.1	Lrp/AsnC ligand binding domain-containing protein	-
D4A92_RS20815	4230860..4231552	+	693	WP_203020081.1	LuxR family transcriptional regulator	-
D4A92_RS20820	4231637..4232746	-	1110	WP_203017038.1	DUF2333 family protein	-
D4A92_RS20825	4232739..4234034	-	1296	WP_203017039.1	hypothetical protein	-
D4A92_RS20830	4234031..4234441	-	411	WP_203017040.1	helix-turn-helix transcriptional regulator	Antitoxin
D4A92_RS20835	4234441..4234701	-	261	WP_203017041.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
D4A92_RS20840	4234753..4236666	-	1914	WP_203017042.1	AAA family ATPase	-
D4A92_RS20845	4236669..4237820	-	1152	WP_203017043.1	hypothetical protein	-
D4A92_RS20850	4237900..4238988	-	1089	WP_203017044.1	hypothetical protein	-
D4A92_RS20855	4239195..4239581	-	387	WP_203017045.1	cytochrome c family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-82)

Antitoxin

• Cro/C1-type helix-turn-helix domain (69-113)

Sequences

Toxin

Protein

• Length: 87 a.a.
• Molecular weight: 9965.39 Da
• Isoelectric Point: 10.8940

>T111754 WP_203017041.1 NZ_CP032405:c4234701-4234441 [Rhizobium rosettiformans]
VRHVVYSKDAIRSLNRMPANEARRIRSKVLQYATEPASLANNVKKLRDSRYHRLRIGDWRVIFREDGTVVDVVRVAARGE
AYEGEL

Nucleotide

• Length: 261 bp

>T111754 NZ_CP032405:c4234701-4234441 [Rhizobium rosettiformans]
GTGCGGCACGTTGTTTACAGCAAGGATGCCATTCGTAGCTTGAACCGGATGCCGGCTAACGAGGCGCGACGTATCCGGTC
GAAGGTTCTTCAGTATGCCACCGAACCGGCTTCACTCGCCAACAATGTCAAAAAGTTACGTGACAGCCGTTACCATCGCC
TGCGGATCGGGGACTGGCGCGTGATCTTCCGTGAAGACGGAACCGTCGTCGACGTGGTGCGTGTCGCAGCACGCGGTGAA
GCCTATGAAGGAGAGCTCTGA

Antitoxin

Protein

• Length: 137 a.a.
• Molecular weight: 15081.99 Da
• Isoelectric Point: 4.3198

>AT111754 WP_203017040.1 NZ_CP032405:c4234441-4234031 [Rhizobium rosettiformans]
MTIASSFTTPNGEEMIVLSRKDYEALIQRIEDLEDSLAVADFERKLAAGQEELIPAEYVNRMGEGESPIRVWRDFRGMAA
KDLAAAAGISTAYLSEIETGKKDGSVATLKAIADVLKLDLDDLYWRHEDRKVTDTA

Nucleotide

• Length: 411 bp

>AT111754 NZ_CP032405:c4234441-4234031 [Rhizobium rosettiformans]
ATGACGATCGCATCATCGTTTACGACGCCAAATGGCGAAGAGATGATCGTACTCAGCCGCAAGGACTATGAAGCACTGAT
CCAGCGCATCGAGGATCTGGAAGACAGTCTGGCTGTCGCCGATTTCGAGAGGAAGCTTGCTGCGGGCCAGGAAGAGCTGA
TCCCTGCCGAGTACGTCAATCGGATGGGTGAAGGTGAAAGCCCGATCCGCGTCTGGCGCGATTTTCGCGGTATGGCGGCC
AAGGACCTTGCTGCCGCTGCCGGAATCAGCACCGCTTATCTGTCCGAGATCGAAACAGGCAAGAAGGACGGCAGTGTCGC
AACACTCAAAGCGATCGCCGATGTCTTGAAGCTCGACCTCGATGACCTCTACTGGCGGCACGAGGACAGGAAGGTCACAG
ATACCGCATGA