Detailed information of TA system TA111752

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT-BrnA
• Location: 2709004..2709560
• Replicon: chromosome
• Accession: NZ_CP032405
• Organism: Rhizobium rosettiformans strain MAE2-X

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: -
• Locus tag: D4A92_RS13115
• Protein ID: WP_203013835.1
• Coordinates: 2709312..2709560 (-)
• Length: 83 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: -
• Locus tag: D4A92_RS13110
• Protein ID: WP_203013833.1
• Coordinates: 2709004..2709315 (-)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D4A92_RS13075	2704403..2704612	-	210	WP_006727165.1	cold-shock protein	-
D4A92_RS13080	2704625..2704906	-	282	WP_203013824.1	hypothetical protein	-
D4A92_RS13085	2705075..2705566	+	492	WP_203013826.1	histidine kinase	-
D4A92_RS13090	2705882..2706934	+	1053	WP_203013828.1	fatty acid desaturase	-
D4A92_RS13095	2707024..2707716	-	693	WP_203013830.1	YafY family transcriptional regulator	-
D4A92_RS13100	2707838..2708473	+	636	WP_203013832.1	LysE family translocator	-
D4A92_RS13105	2708483..2708974	-	492	WP_203019954.1	GNAT family N-acetyltransferase	-
D4A92_RS13110	2709004..2709315	-	312	WP_203013833.1	BrnA antitoxin family protein	Antitoxin
D4A92_RS13115	2709312..2709560	-	249	WP_203013835.1	BrnT family toxin	Toxin
D4A92_RS13120	2709621..2709941	-	321	WP_203013837.1	YnfA family protein	-
D4A92_RS13125	2709992..2710810	-	819	WP_203013838.1	EAL domain-containing protein	-
D4A92_RS13130	2710952..2712520	-	1569	WP_203013840.1	DUF853 domain-containing protein	-
D4A92_RS13135	2712747..2713139	+	393	WP_076395111.1	DUF937 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (5-78)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (28-101)

Sequences

Toxin

Protein

• Length: 83 a.a.
• Molecular weight: 9132.75 Da
• Isoelectric Point: 10.5863

>T111752 WP_203013835.1 NZ_CP032405:c2709560-2709312 [Rhizobium rosettiformans]
MIIVWDEPKRLQNLAKHGLDFAELDERFFVGASIRPAKSGRTIAIGELNGRIVVAVIYMPLGPEALSIISMRAASRRERS
LI

Nucleotide

• Length: 249 bp

>T111752 NZ_CP032405:c2709560-2709312 [Rhizobium rosettiformans]
ATGATCATCGTCTGGGACGAACCGAAGCGGCTGCAGAATCTGGCAAAGCACGGCCTCGACTTTGCCGAGCTCGATGAGCG
CTTCTTCGTCGGTGCCAGCATCCGTCCGGCAAAATCGGGCCGGACTATCGCGATCGGTGAGCTGAACGGTCGTATCGTTG
TTGCAGTGATCTATATGCCTTTGGGCCCCGAAGCGCTGTCGATCATTTCCATGCGCGCGGCCAGCCGGCGGGAGAGGAGC
CTCATATGA

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11704.23 Da
• Isoelectric Point: 4.6969

>AT111752 WP_203013833.1 NZ_CP032405:c2709315-2709004 [Rhizobium rosettiformans]
MSGKFSSKRPLTDEEEAEIQAMIASDPDNPEITEEELKEFRPFREVFPDLAEAIDRKLGRPKAESPKKAISIRLDQEVID
RFKATGDGWQSRMNEALRKAVGL

Nucleotide

• Length: 312 bp

>AT111752 NZ_CP032405:c2709315-2709004 [Rhizobium rosettiformans]
ATGAGCGGGAAGTTTTCGTCCAAGAGACCGCTGACAGATGAAGAAGAGGCGGAGATCCAGGCGATGATCGCGTCCGATCC
CGACAATCCGGAAATCACAGAAGAAGAGTTGAAAGAGTTCCGCCCCTTCCGTGAGGTCTTTCCGGATCTCGCCGAGGCAA
TAGACCGCAAGCTCGGCCGGCCGAAGGCAGAGAGCCCAAAAAAGGCGATCAGCATCCGGCTCGATCAGGAGGTGATCGAC
CGCTTCAAGGCAACAGGCGACGGCTGGCAGTCGCGGATGAACGAGGCCTTGCGCAAGGCGGTGGGGCTGTGA