Detailed information of TA system TA111748

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parE-PrpA/ParE-RHH
• Location: 1030374..1030911
• Replicon: chromosome
• Accession: NZ_CP032405
• Organism: Rhizobium rosettiformans strain MAE2-X

Toxin (Protein)

• Gene name: parE
• Uniprot ID: -
• Locus tag: D4A92_RS04955
• Protein ID: WP_203018493.1
• Coordinates: 1030374..1030664 (-)
• Length: 97 a.a.

Antitoxin (Protein)

• Gene name: PrpA
• Uniprot ID: K2QBR4
• Locus tag: D4A92_RS04960
• Protein ID: WP_006724430.1
• Coordinates: 1030666..1030911 (-)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D4A92_RS04940	1027262..1027690	+	429	WP_203018490.1	hypothetical protein	-
D4A92_RS04945	1027799..1028989	+	1191	WP_203018491.1	multidrug effflux MFS transporter	-
D4A92_RS04950	1029044..1030267	-	1224	WP_203018492.1	argininosuccinate synthase	-
D4A92_RS04955	1030374..1030664	-	291	WP_203018493.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
D4A92_RS04960	1030666..1030911	-	246	WP_006724430.1	type II toxin-antitoxin system ParD family antitoxin	Antitoxin
D4A92_RS04965	1031014..1031877	+	864	WP_203018495.1	SDR family NAD(P)-dependent oxidoreductase	-
D4A92_RS04970	1031952..1032593	+	642	WP_203018497.1	LysE family translocator	-
D4A92_RS04975	1032627..1033865	-	1239	WP_203018499.1	23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN	-
D4A92_RS04980	1034101..1034604	-	504	WP_203019837.1	hypothetical protein	-
D4A92_RS04985	1034868..1035248	-	381	WP_203018501.1	DUF1232 domain-containing protein	-
D4A92_RS04990	1035318..1035620	-	303	WP_203018503.1	4a-hydroxytetrahydrobiopterin dehydratase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-91)

Antitoxin

• Antitoxin ParD (1-78)

Sequences

Toxin

Protein

• Length: 97 a.a.
• Molecular weight: 11334.08 Da
• Isoelectric Point: 10.1634

>T111748 WP_203018493.1 NZ_CP032405:c1030664-1030374 [Rhizobium rosettiformans]
MAAYRLSPQARRDLEGIWRYSWERWSKDQADHYYMAIIEAFEDLEAGRKAGRPLAIAKRGYLSLACGSHFIIYAQRKQTV
EIIRILHQRMNIGRHL

Nucleotide

• Length: 291 bp

>T111748 NZ_CP032405:c1030664-1030374 [Rhizobium rosettiformans]
ATGGCTGCCTACAGGCTTTCACCGCAAGCGCGGAGGGATCTTGAAGGCATATGGCGATATTCTTGGGAGCGTTGGTCTAA
AGACCAGGCCGACCACTATTACATGGCGATCATCGAAGCGTTCGAAGATCTTGAGGCCGGACGAAAGGCTGGCAGACCCT
TGGCTATCGCCAAGCGCGGCTATCTCAGTCTCGCCTGTGGATCCCACTTTATCATCTACGCGCAGCGAAAGCAGACTGTT
GAAATTATCCGCATCCTCCACCAGCGCATGAATATCGGTCGCCATCTCTGA

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 8655.62 Da
• Isoelectric Point: 3.9302

>AT111748 WP_006724430.1 NZ_CP032405:c1030911-1030666 [Rhizobium rosettiformans]
MNKVTTVTVSGELAEFIEAKISAGEYASEAEVIEAALALLRDEVDGLAAIQAAIEEGEASGEPQPFDPRRFLDEMHSKYG
A

Nucleotide

• Length: 246 bp

>AT111748 NZ_CP032405:c1030911-1030666 [Rhizobium rosettiformans]
ATGAACAAGGTTACGACCGTCACGGTCTCCGGGGAACTGGCGGAGTTCATCGAAGCCAAGATTTCTGCCGGGGAGTATGC
CTCGGAAGCCGAGGTCATTGAGGCCGCGCTCGCCCTGTTGCGCGATGAGGTCGATGGTCTTGCCGCTATCCAGGCGGCCA
TTGAGGAGGGCGAGGCCTCTGGTGAGCCGCAGCCGTTTGACCCCAGGCGGTTTCTCGACGAAATGCACAGCAAATACGGA
GCCTGA

Structures

Antitoxin

• AlphaFold DB: K2QBR4