Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) VapXD/-
Location 311..774 Replicon plasmid pTFGA2
Accession NZ_CP032399
Organism Neisseria gonorrhoeae strain TFG-A2

Toxin (Protein)


Gene name VapD Uniprot ID Q51003
Locus tag TFGA2_RS13045 Protein ID WP_003690225.1
Coordinates 496..774 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name VapX Uniprot ID Q51002
Locus tag TFGA2_RS13040 Protein ID WP_003690223.1
Coordinates 311..496 (+) Length 62 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
TFGA2_RS13035 63..308 + 246 WP_003690221.1 hypothetical protein -
TFGA2_RS13040 311..496 + 186 WP_003690223.1 DUF5397 family protein Antitoxin
TFGA2_RS13045 496..774 + 279 WP_003690225.1 endoribonuclease VapD Toxin
TFGA2_RS13050 752..1021 - 270 WP_003692166.1 hypothetical protein -
TFGA2_RS13055 1053..1694 - 642 WP_003690229.1 Cryptic plasmid protein B -
TFGA2_RS13060 1796..2428 - 633 WP_082287524.1 relaxase/mobilization nuclease domain-containing protein -
TFGA2_RS13065 2413..2604 - 192 WP_003690218.1 plasmid mobilization relaxosome protein MobC -
TFGA2_RS13465 2872..3096 + 225 WP_025455920.1 hypothetical protein -
TFGA2_RS13075 3266..4048 + 783 WP_010359605.1 replication initiation protein -
TFGA2_RS13080 4052..4294 + 243 WP_003690220.1 HTH domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..4305 4305


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Virulence-associated protein D / CRISPR associated protein Cas2

(1-86)

Antitoxin

Protein of unknown function DUF5397

(2-60)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10473.89 Da        Isoelectric Point: 6.9726

>T111744 WP_003690225.1 NZ_CP032399:496-774 [Neisseria gonorrhoeae]
MYAISFDLVVADTAQNHPKGISQAYADIGYTLRKFGFTRIQGSLYTCQNEDMANLFSAINELKALPWFPSSVRDIRAFRI
EQWSDFTSLVKS

Download         Length: 279 bp

>T111744 NZ_CP032399:496-774 [Neisseria gonorrhoeae]
ATGTACGCGATTTCTTTTGATTTGGTGGTTGCCGATACCGCTCAAAACCATCCGAAAGGCATTTCCCAAGCCTACGCGGA
TATTGGATACACCCTGCGGAAATTCGGCTTTACCCGCATTCAGGGAAGTTTGTACACCTGCCAAAACGAAGATATGGCTA
ACCTGTTTTCAGCCATCAACGAGCTAAAAGCTCTGCCTTGGTTTCCGTCTTCTGTCCGCGATATTCGGGCGTTCCGCATT
GAGCAGTGGTCTGATTTCACAAGTCTTGTGAAGTCTTAA

Antitoxin


Download         Length: 62 a.a.        Molecular weight: 6717.54 Da        Isoelectric Point: 4.2641

>AT111744 WP_003690223.1 NZ_CP032399:311-496 [Neisseria gonorrhoeae]
MQTTIPTGSIRTFGDYGVMYIVGTPAEQLPDGDWLVNIELPESGEHTQYKLSHIVQDPKAA

Download         Length: 186 bp

>AT111744 NZ_CP032399:311-496 [Neisseria gonorrhoeae]
ATGCAAACCACTATCCCCACCGGTTCAATCCGCACCTTTGGCGATTACGGCGTGATGTACATCGTCGGCACGCCTGCCGA
ACAGCTTCCGGACGGCGATTGGCTCGTAAATATTGAACTGCCGGAAAGCGGCGAACACACGCAATACAAACTGTCCCACA
TCGTTCAAGACCCAAAGGCTGCCTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7H5FJI5

Antitoxin

Source ID Structure
AlphaFold DB Q51002

References