Detailed information of TA system TA111731

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 25249..25774
• Replicon: plasmid p22453_2
• Accession: NZ_CP032394
• Organism: Salmonella enterica subsp. enterica serovar Dublin strain CVM 22453

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: I3W3D5
• Locus tag: D5G00_RS24975
• Protein ID: WP_001159863.1
• Coordinates: 25469..25774 (+)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: M7S5D0
• Locus tag: D5G00_RS24970
• Protein ID: WP_000813641.1
• Coordinates: 25249..25467 (+)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D5G00_RS24925	20276..20989	+	714	WP_000801115.1	hypothetical protein	-
D5G00_RS24935	21114..21698	+	585	WP_001575501.1	hypothetical protein	-
D5G00_RS24940	21771..21983	+	213	WP_000063794.1	hypothetical protein	-
D5G00_RS26455	22244..22405	+	162	WP_001816720.1	hypothetical protein	-
D5G00_RS24945	22431..23279	+	849	WP_000175391.1	SdiA-regulated domain-containing protein	-
D5G00_RS24960	23849..24277	-	429	WP_000812999.1	type II toxin-antitoxin system VapC family toxin	-
D5G00_RS24965	24274..24504	-	231	WP_001261283.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
D5G00_RS24970	25249..25467	+	219	WP_000813641.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
D5G00_RS24975	25469..25774	+	306	WP_001159863.1	type II toxin-antitoxin system toxin CcdB	Toxin
D5G00_RS24985	25844..26548	-	705	WP_001067858.1	IS6-like element IS26 family transposase	-
D5G00_RS24990	26826..27161	+	336	WP_001527010.1	hypothetical protein	-
D5G00_RS24995	27218..27784	+	567	WP_001541562.1	DUF2913 family protein	-
D5G00_RS25000	27816..28758	-	943	Protein_33	Rpn family recombination-promoting nuclease/putative transposase	-
D5G00_RS25005	29151..29771	+	621	WP_000864788.1	ParA family protein	-
D5G00_RS25010	29823..30053	+	231	WP_000051066.1	partitioning protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..55415	55415

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (1-125)

Antitoxin

• Antitoxin VapB-like (2-81)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 13091.77 Da
• Isoelectric Point: 12.8043

>T111731 WP_001159863.1 NZ_CP032394:25469-25774 [Salmonella enterica subsp. enterica serovar Dublin]
KKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKK
KKKKKKKKKKKKKKKKKKKKKK

Nucleotide

• Length: 306 bp

>T111731 NZ_CP032394:25469-25774 [Salmonella enterica subsp. enterica serovar Dublin]
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 9374.72 Da
• Isoelectric Point: 12.6573

>AT111731 WP_000813641.1 NZ_CP032394:25249-25467 [Salmonella enterica subsp. enterica serovar Dublin]
KKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKK

Nucleotide

• Length: 219 bp

>AT111731 NZ_CP032394:25249-25467 [Salmonella enterica subsp. enterica serovar Dublin]
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA

Structures

Toxin

• AlphaFold DB: I3W3D5

Antitoxin

• AlphaFold DB: A0A656ICA6