Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 244409..245051 Replicon chromosome
Accession NZ_CP032365
Organism Bacillus wiedmannii strain SR52

Toxin (Protein)


Gene name pemK Uniprot ID J8CWW5
Locus tag D4A37_RS01385 Protein ID WP_000635963.1
Coordinates 244701..245051 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID R8I8H2
Locus tag D4A37_RS01380 Protein ID WP_000004570.1
Coordinates 244409..244696 (+) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
D4A37_RS01355 239725..240687 + 963 WP_000961179.1 UV DNA damage repair endonuclease UvsE -
D4A37_RS01360 240680..241252 - 573 WP_000906928.1 rhomboid family intramembrane serine protease -
D4A37_RS01365 241345..241704 + 360 WP_000635044.1 holo-ACP synthase -
D4A37_RS01370 241861..242811 + 951 WP_002096914.1 outer membrane lipoprotein carrier protein LolA -
D4A37_RS01375 242930..244099 + 1170 WP_000390587.1 alanine racemase -
D4A37_RS01380 244409..244696 + 288 WP_000004570.1 antitoxin EndoAI Antitoxin
D4A37_RS01385 244701..245051 + 351 WP_000635963.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
D4A37_RS01390 245120..247288 + 2169 WP_064459811.1 RNA-binding transcriptional accessory protein -
D4A37_RS01395 247347..247463 - 117 WP_001143642.1 cortex morphogenetic protein CmpA -
D4A37_RS01400 247659..248117 + 459 WP_000344253.1 SprT family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12974.12 Da        Isoelectric Point: 5.7168

>T111711 WP_000635963.1 NZ_CP032365:244701-245051 [Bacillus wiedmannii]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDEVMMIRVDEALQISLGLIDF

Download         Length: 351 bp

>T111711 NZ_CP032365:244701-245051 [Bacillus wiedmannii]
TTGATTGTAAAACGCGGCGACGTGTATTTTGCAGACCTTTCCCCAGTTGTTGGTTCTGAGCAAGGAGGCGTTCGTCCGGT
TCTTGTCATTCAAAATGACATCGGAAATCGTTTTAGTCCAACGGTGATTGTAGCGGCTATTACTGCACAGATTCAAAAAG
CTAAATTACCCACTCATGTGGAAATTGATGCGAAAAAGTACGGTTTTGAGAGAGATTCTGTTATTTTACTTGAGCAGATT
CGAACAATCGATAAGCAGCGCTTAACGGACAAAATCACTCACTTAGATGAAGTGATGATGATTCGTGTAGATGAAGCGCT
ACAAATTAGTTTAGGACTAATAGATTTTTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10780.36 Da        Isoelectric Point: 8.9605

>AT111711 WP_000004570.1 NZ_CP032365:244409-244696 [Bacillus wiedmannii]
VSESSVTTEIVVRLPKQMVTELDGIGKQENKNRHELICQATQLLLRQHKTKKRYQHESMRRGYIEMGKINLGIASEAFLA
EYEAAHTVERLVSGG

Download         Length: 288 bp

>AT111711 NZ_CP032365:244409-244696 [Bacillus wiedmannii]
GTGTCCGAATCAAGTGTAACTACTGAAATCGTGGTTCGGTTGCCAAAGCAAATGGTAACGGAATTGGACGGAATTGGAAA
ACAAGAGAATAAGAATCGCCATGAACTAATTTGCCAGGCAACACAACTGTTATTGCGTCAACATAAGACGAAGAAACGCT
ACCAACATGAATCAATGCGACGTGGGTACATTGAAATGGGAAAAATTAATCTTGGTATTGCATCGGAAGCTTTCTTAGCA
GAGTATGAAGCAGCTCATACAGTAGAACGCTTAGTTAGCGGGGGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4HKE
PDB 7BXY

Antitoxin

Source ID Structure
AlphaFold DB A0A366FY90

References